Decoding Cortical Glial Cell Development  

作　　者：Xiaosu Li Guoping Liu Lin Yang Zhenmeiyu Li Zhuangzhi Zhang Zhejun Xu Yuqun Cai Heng Du Zihao Su Ziwu Wang Yangyang Duan Haotian Chen Zicong Shang Yan You Qi Zhang Miao He Bin Chen Zhengang Yang 

机构地区：[1]State Key Laboratory of Medical Neurobiology,Institutes of Brain Science,MOE Frontiers Center for Brain Science,Department of Neurology,Institute for Translational Brain Research,Zhongshan Hospital,Fudan University,Shanghai 200032,China [2]Department of Molecular,Cell and Developmental Biology,University of California Santa Cruz,Santa Cruz,CA 95064,USA

出　　处：《Neuroscience Bulletin》2021年第4期440-460,共21页神经科学通报（英文版）

基　　金：supported by grants from the National Key Research and Development Program of China(2018YFA0108000);the National Natural Science Foundation of China(31630032,31820103006,and 32070971);a Shanghai Municipal Science and Technology Major Project(2018SHZDZX01);ZJLab,and grants from NIH(R01MH094589 and R01NS089777)。

摘　　要：Mouse cortical radial glial cells(RGCs)are primary neural stem cells that give rise to cortical oligodendrocytes,astrocytes,and olfactory bulb(OB)GABAergic interneurons in late embryogenesis.There are fundamental gaps in understanding how these diverse cell subtypes are generated.Here,by combining single-cell RNA-Seq with intersectional lineage analyses,we show that beginning at around E16.5,neocortical RGCs start to generate ASCL1^(+)EGFR^(+)apical multipotent intermediate progenitors(MIPCs),which then differentiate into basal MIPCs that express ASCL1,EGFR,OLIG2,and MKI67.These basal MIPCs undergo several rounds of divisions to generate most of the cortical oligodendrocytes and astrocytes and a subpopulation of OB interneurons.Finally,single-cell ATAC-Seq supported our model for the genetic logic underlying the specification and differentiation of cortical glial cells and OB interneurons.Taken together,this work reveals the process of cortical radial glial cell lineage progression and the developmental origins of cortical astrocytes and oligodendrocytes.

关 键 词：Radial glial cell Intermediate progenitor cell ASCL1 EGFR OLIG2 OLIGODENDROCYTE ASTROCYTE Olfactory bulb interneuron Cerebral cortex 

分 类 号：Q421[生物学—神经生物学]