肺结核合并糖尿病患者发生乙胺丁醇耐药的基因突变研究  

Ethambutol-resistant gene mutations in patients with tuberculosis and diabetes

作　　者：陈晶 马庆庆[2] 龚亚东[2] 陈云华 唐竹 林牧[2] CHEN Jing;MA Qingqing;GONG Yadong;CHEN Yunhua;TANG Zhu;LIN Mu(Zunyi Medical College,Zunyi,Guizhou 563006,China;The Central Laboratory,Guizhou Aerospace Hospital,Zunyi,Guizhou 563000,China)

机构地区：[1]遵义医药高等专科学校,贵州遵义563006 [2]贵州航天医院中心实验室,贵州遵义563000

出　　处：《现代医药卫生》2021年第10期1641-1643,1647,共4页Journal of Modern Medicine & Health

基　　金：遵义市科技局科技计划项目[遵市科合支撑NS(2019)16号];遵义市汇川区科技局计划项目[遵汇科合(2020)8号]。

摘　　要：目的了解肺结核(TB)合并糖尿病(TB-DM)患者发生乙胺丁醇耐药的相关基因分子特征。方法通过TB-DNA、TR-RNA和抗酸染色(结果均为阳性)筛选出2017年12月至2019年7月该院就诊的763例结核病患者,将其分为TB组和TB-DM组,经PCR-反向点杂交法检测耐药相关基因embB,对比分析2组基因突变特点,并搜集相关临床资料以探讨其与乙胺丁醇耐药发生的相关性。结果TB组的525例患者中19例发生乙胺丁醇耐药,TB-DM组的207例患者中13例发生乙胺丁醇耐药。2组发生乙胺丁醇耐药突变时,embB306N突变率(>84.62%)最高。结论TB组乙胺丁醇耐药患者易发生embB306N和embB306M2位点突变,TB-DM组易发生embB306M1位点突变。Objective To understand the molecular characteristics of genes related to ethambutol-resistance in patients with tuberculosis(TB)and diabetes(TB-DM).Methods A total of 763 patients with TB admitted to the hospital from December 2017 to July 2019 were screened by TB-DNA,TR-RNA,and acid-fast staining(all were positive).The patients were divided into TB group and TB-DM group.The ethambutol related gene embB characteristics of gene mutations in the two groups were compared and analyzed by PCR-reverse dot hybridization.The clinical data were collected to explore its correlation with the occurrence of ethambutol-resistance.Results Nineteen of the 525 patients in the TB group developed ethambutol-resistance,and 13 of the 207 patients in the TB-DM group developed ethambutol-resistance.The embB306N mutation rate(>84.62%)was the highest.Conclusion The embB306N and embB306M2 mutations in TB patients with ethambutol-resistance are prone to occur,the embB306M1 mutation is prone to occur in the TB-DM patients with ethambutol-resistance.

关 键 词：肺结核 糖尿病 乙胺丁醇耐药 基因突变 

分 类 号：R521[医药卫生—内科学] R587.1[医药卫生—临床医学]