核因子κB与糖皮质激素受体在口腔扁平苔藓患者口腔黏膜组织中的表达及其意义  

Expression and Correlation of NF-κB with Glucocorticoid Receptor of Oral Mucosa in Patients with Oral Lichen Planus

作　　者：杨绣川 黎芳 孙燕[2] YANG Xiu-chuan;LI Fang;SUN Yan(Department of Stomatology,Anting Hospital,Shanghai Jiading District,Shanghai 201805)

机构地区：[1]上海市嘉定区安亭医院口腔科,上海201805 [2]上海交通大学医学院附属第九人民医院口腔科,上海200011

出　　处：《医学临床研究》2021年第4期493-496,共4页Journal of Clinical Research

基　　金：上海市卫生和计划生育委员会科研课题(编号:20184Y0189)。

摘　　要：[目的]探讨核因子κB(nuclear factor-κB,NF-κB)、糖皮质激素受体(glucocorticoid receptor,GR)在口腔扁平苔藓(oral lichen planus,OLP)患者口腔黏膜组织中的表达及其意义。[方法]选取2017年2月至2019年2月在上海市嘉定区安亭医院诊治的口腔扁平苔藓患者局部病损口腔黏膜标本126例(观察组),另外选取在上海市嘉定区安亭医院门诊行阻生牙拔除术需切除的健康口腔黏膜(60例),采用实时定量反转录PCR(reverse transcription PCR,RT PCR)与蛋白质印迹法检测两组患者黏膜中NF-κB的亚型p50、p65以及GR的亚型糖皮质激素受体β(GRβ)、糖皮质激素受体a(GRa)表达水平,并分析NF-κB与GR的相关性。[结果]观察组在牙龈、舌、颊等区域黏膜均有病损,主要损伤类型包括糜烂型以及斑纹型;病理表现:固有层淋巴细胞带状漫润,基底细胞排列紊乱,上皮钉突不规则延长,棘层增生,上皮不全角化。两组口腔黏膜中p50、p65,GRB.GRa均有表达,其中观察组中GRa蛋白表达减少,p50、p65表达增加,GRβ无显著变化。观察组中GRa mRNA表达水平显著低于对照组,差异具有统计学意义(P<0.05);观察组中p50、p65表达水平显著高于对照组的,差异具有统计学意义(P<0.05),但两组中GRβmRNA水平比较差异无统计学意义(P>0.05)。在口腔扁平苔藓患者黏膜中,p50、p65与GRa均呈负相关(r=-0.536,P=0.002;r=-0.428,P=0.015)。[结论]口腔扁平苔藓患者的GRa水平降低,p50、p65表达增加,在该疾病的炎症反应中NF-κB与GR有拮抗作用。[Objective]To study the expression and correlation of nuclear factor-κB(NF-κb)with glu-cocorticoid receptor(GR)of oral mucosa in patients with oral lichen planus(OLP).[Methods]A total of 126 patients with oral lichen planus who were treated in Department of Stomatology,Anting Hospital,Shanghai Jiading District from February 2017 to February 2019 were selected as eclinical research objects(the observation group)for retrospective analysis.And 60 healthy oral mucosa extracted from the oral mucosa,which required to be removed for extraction of impacted teeth in outpatient department,were selected as the control group.The expression of the subunits of NF-κb including P50 and p65,and the subtypes of GR including GRβ(glu-cocorticoid receptorβ)and GR a(glucocorticoid receptor a)in the mucosa of the two groups were analyzed by RT-PCR and w estern blotting,and the correlation between NF-κB and GR in OLP was explored.[Results]There were lesions in gingiva.tongue.buccal and other areas in the observation group.The main types of le-sions were erosive and mottled.The main pathological manifestations were:banded infiltration of lymphocytes in lamina propria,disorder of basal cell arrangement,rregular prolongation of epithelial nail process,acantho-sis,epithelial hyperkeratosis.The expressionsof NF-κB P50,p65,GRβand GR a were shown in the oral mucosa of the two groups.Among them,the expression of GR a protein in the observation group decreased,while the expression of P50 and p65 increased.However,there was no significant change in GRβ.The expres-sion level of GR a mRNA in the observation group was significantly lower than that in the control group,the difference was statistically significant(P<0.05);the expression leve!of P50 and p65 in the observation group was significantly higher than that in the control group,the difference was statistically significant(P<0.05);there was no significant difference in GRβmRNA level between the two groups,the difference was not statistically significant(P>0.05).In the mucosa of patien

关 键 词：扁平苔癣 口腔/病理学 NF-κB 受体 糖皮质激素 口腔黏膜 

分 类 号：R781.59[医药卫生—口腔医学]