高效苯酚降解菌Bacillus sp. L5-1的分离及其降解特性  

Isolation and degradation characteristics of highly efficient phenol-degrading bacteria Bacillus sp.L5-1

作　　者：刘庆辉 李剑[1] 杨航 王志宇 李艳[1] 张玮川 贾银娟 张秋根[1] 罗旭彪[1] LIU Qing-hui;LI Jian;YANG Hang;WANG Zhi-yu;LI Yan;ZHANG Wei-chuan;JIA Yin-juan;ZHANG Qiu-gen;LUO Xu-biao(National-Local Joint Engineering Research Center of Heavy Metals Pollutants Control and Resource Utilization,Nanchang Hangkong University,Nanchang 330063,China)

机构地区：[1]南昌航空大学,重金属污染物控制与资源化国家地方联合工程研究中心,江西南昌330063

出　　处：《中国环境科学》2021年第5期2441-2448,共8页China Environmental Science

基　　金：国家自然科学基金资助项目(21467018);江西省教育厅项目(GJJ170576);江西省重点研发计划项目(20181ACG70021)。

摘　　要：从污水处理厂活性污泥中分离筛选出一株高效苯酚降解菌L5-1,经菌落形态观察和16S rDNA基因测序,结果表明菌株L5-1为蜡样芽胞杆菌(Bacillus cereus),美国国家生物信息中心(NCBI)的注册号为MN784421.将苯酚设置为唯一碳源,对其生长和苯酚降解特性展开研究.结果表明:菌株L5-1在10%接种量、温度30~35℃、pH值7~8的条件下,均能高效降解培养基中苯酚(培养基体积为100mL,初始苯酚浓度为500mg/L,14h时降解率>93%).而在最优降解条件下(10%接种量,培养温度为35℃,pH值7.0,NaCl浓度为1%),初始苯酚浓度为500mg/L,菌株在14h内的苯酚降解率可达97.1%;而当初始苯酚浓度为1000mg/L,菌株也可在46h内达到97.71%的降解率.运用Haldance方程动力学模拟菌株在不同浓度苯酚下的生长过程,其最大比生长速率为0.355h^(-1),半饱合常数104.27mg/L,抑制常数为322.83mg/L,R^(2)=0.997.菌株L5-1为目前已报道的Bacillus菌属中降解苯酚能力较强的菌株,为实际处理含酚废水中提供理论参考.A highly efficient phenol-degrading bacterium named L5-1 was isolated and screened from the activated sludge from a sewage treatment plant.The colony morphology observation and 16S rDNA gene sequencing showed that the strain L5-1 was Bacillus cereus,with the registration number of MN784421 in the US National Center for Biotechnology Information(NCBI).A series of experiments with Phenol as the only carbon source were conducted to study the growth and phenol degradation characteristics of this strain L5-1.The results showed that under the conditions of 10%inoculum,temperature range of 30 to 35℃,pH range of 7 to 8,the strain L5-1 effectively degraded phenol in the culture medium(with the 100mL of medium volume and the initial phenol concentration of 500mg/L),the degradation rate was better than 93%in 14h.Under optimal degradation conditions(10%inoculum,culture temperature at 35℃,pH 7.0,and NaCl concentration at 1%),The phenol degradation rate reached 97.1%within 14 hours when the initial concentration was set at 500mg/L.When the initial phenol concentration was set to 1000mg/L,the strain L5-1 still reached 97.71%degradation rate within 46 hours.The Haldane kinetic model was used to simulate the growth process of strains under different concentrations of phenol.The maximum specific growth rate was 0.355h^(-1),the semi-saturation constant was 104.27mg/L,the inhibition constant was 322.83mg/L,R^(2)=0.997.This study confirmed Strain L5-1 was a Bacillus strains with strong phenol degradation ability among the reported strains of the genus Bacillus,and provided certain theoretical references for the actual treatment of phenol-containing wastewater.

关 键 词：Bacillus cereus 苯酚 生物降解 动力学 

分 类 号：X172[环境科学与工程—环境科学]