碳化硼促进Psendomonas stutzeri A1501电催化固氮产氨及机制  

Boron carbide promotes the ammonia production by electrocatalytic nitrogen fixation with Psendomonas Stutzeri A1501

作　　者：董国文 陈飘[1] 任国平 王超 金曙光 叶捷 周顺桂 DONG Guo-wen;CHEN Piao;REN Guo-ping;WANG Chao;JIN Shu-guang;YE Jie;ZHOU Shun-gui(Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation,College of Resources and Environment,Fujian Agriculture and Forestry University,Fuzhou 350002,China;College of Resources and Chemical Engineering,Sanming University,Sanming 365004,China;Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China)

机构地区：[1]福建农林大学资源与环境学院,福建省土壤健康与调查重点实验室,福建福州350002 [2]三明学院资源与化工学院,福建三明365004 [3]中国科学院生态环境研究中心,北京100085

出　　处：《中国环境科学》2021年第5期2449-2458,共10页China Environmental Science

基　　金：国家自然科学基金资助项目(41977281)。

摘　　要：以Pseudomonas stutzeri(P.stutzeri)A1501为模式固氮菌株、碳化硼(B_(4)C)为典型电子传递载体构建电催化氮还原体系,探究B_(4)C添加对该体系固氮产氨性能的影响.结果表明,B_(4)C能够显著提高P.stutzeri A1501介导的电催化氮还原体系的产氨性能.在0.4g/L投加量条件下,900℃煅烧温度下制备的B_(4)C(B_(4)C_(900))的添加能够使电催化体系氨累积量提高117%,这是由于B_(4)C_(900)表面丰富的活化位点能够促进N2吸附与活化.此外,电化学和光谱学谱图表明,B_(4)C_(900)的添加不仅能够促使P.stutzeri A1501分泌更多的氧化还原活性物质,而且驱动高活性阴极生物膜的形成,进而强化电极电子的长距离传递及利用.Pseudomonas stutzeri(P.stutzeri)A1501was used as a model nitrogen-fixing strain and boron carbide(B_(4)C)was selected as a typical electron transfer carrier to construct an electrocatalytic nitrogen reduction system.The effect of B_(4)C addition on the ammonia production under different conditions was explored.The results showed that B_(4)C significantly enhanced the ammonia production of the electrocatalytic nitrogen reduction system mediated by P.stutzeri A1501.With the dosage of 0.4g/L,the addition of B_(4)C(B_(4)C_(900))prepared at 900℃calcination temperature improved the ammonia yield by 117%.This was because the abundant activation sites on the surface of B_(4)C_(900) promote N2 adsorption and activation.In addition,the electrochemical and spectroscopic spectrograms showed that the addition of B_(4)C_(900) not only triggered P.stutzeri A1501 to secrete more redox active substances,but also drove the construction of highly active cathode biofilms,thereby enhancing the long-distance transfer and utilization of electrode electrons.

关 键 词：Psendomonas stutzeri A1501 B_(4)C 电催化 生物固氮 

分 类 号：X172[环境科学与工程—环境科学]