低频超声微泡增强紫杉醇诱导三阴性乳腺癌细胞自噬的作用及机制  

Effect and mechanism of low-frequency ultrasound microbubbles on paclitaxel-induced autophagy in triple negative breast cancer cells

作　　者：李颖[1] 董明华[1] 张萌萌 孟芳 杨晓慧[2] LI Ying;DONG Minghua;ZHANG Mengmeng(Department of Ultrasonography,Hengshui City Second People’s Hospital,Hebei,Hengshui 053000,China)

机构地区：[1]河北衡水市第二人民医院超声科,053000 [2]河北衡水市第二人民医院肿瘤科,053000

出　　处：《临床外科杂志》2021年第3期261-264,共4页Journal of Clinical Surgery

摘　　要：目的探讨低频超声微泡增强紫杉醇诱导三阴性乳腺癌细胞自噬的作用及机制。方法将MDA-MB-468细胞分为对照组(不进行处理)、紫杉醇组(6μg/ml紫杉醇)、低频超声组(低频超声+6μg/ml紫杉醇)和低频超声微泡组(低频超声+6μg/ml紫杉醇+微泡),检测各组MDA-MB-468细胞增殖情况,凋亡情况,自噬情况,Bcl-2、Bax、Beclin1、LC3Ⅰ、LC3Ⅱ蛋白表达情况。结果与0μg/ml相比,1、3、6、12、24μg/ml紫杉醇处理下MDA-MB-468细胞增殖抑制率均升高(P<0.05)。与对照组相比,紫杉醇组MDA-MB-468细胞增殖抑制率、凋亡率、细胞中Bax、Beclin1、LC3Ⅱ蛋白表达水平升高(P<0.05),细胞中绿色点状物明显增多,细胞中Bcl-2、LC3Ⅰ蛋白表达水平降低(P<0.05),与紫杉醇组相比,低频超声组MDA-MB-468细胞增殖抑制率、凋亡率、细胞中Bax、Beclin1、LC3Ⅱ蛋白表达水平升高(P<0.05),细胞中绿色点状物明显增多,细胞中Bcl-2、LC3Ⅰ蛋白表达水平降低(P<0.05),与低频超声组相比,低频超声微泡组MDA-MB-468细胞增殖抑制率、凋亡率、细胞中Bax、Beclin1、LC3Ⅱ蛋白表达水平升高(P<0.05),细胞中绿色点状物明显增多,Bcl-2、LC3Ⅰ蛋白表达水平降低(P<0.05)。结论低频超声微泡可通过上调Bax、Beclin1、LC3Ⅱ蛋白表达及下调Bcl-2、LC3Ⅰ蛋白表达增强紫杉醇诱导三阴性乳腺癌细胞的凋亡、自噬反应。Objective To investigate the effect and mechanism of low-frequency ultrasound microbubbles on paclitaxel-induced autophagy in triple negative breast cancer cells. Methods MDA-MB-468 cells were divided into control group( treatment),paclitaxel group( 6 μg/ml paclitaxel),lowfrequency ultrasound group( low-frequency ultrasound + 6 μg/ml paclitaxel) and low-frequency ultrasound microbubbles group( low-frequency ultrasound + 6 μg/ml paclitaxel + microbubbles),the proliferation,apoptosis,autophagy of MDA-MB-468 cells and the expression of Bcl-2,Bax,Beclin1,LC3Ⅰ and LC3Ⅱin MDA-MB-468 cells. Results Compared with 0 μg/ml,1,3,6,12,24 μg/ml paclitaxel increased the proliferation inhibition rate of MDA-MB-468 cells( P < 0. 05). Compared with those in the control group,the proliferation inhibition rate and apoptosis rate of MDA-MB-468 cells,protein expression levels of Bax,Beclin1 and LC3Ⅱ in paclitaxel group were higher( P < 0. 05),the number of green dots was more,and the protein expression levels of Bcl-2 and LC3 were lower( P < 0. 05);compared with those in paclitaxel group,the proliferation inhibition rate and apoptosis rate of MDA-MB-468 cells,protein expression levels of Bax,Beclin1 and LC3 Ⅱ in low-frequency ultrasound group were higher( P < 0. 05),the number of green dots was more,and the protein expression levels of Bcl-2 and LC3 were lower( P < 0. 05);compared with the those in low-frequency ultrasound group,the proliferation inhibition rate and apoptosis rate of MDA-MB-468 cells,protein expression levels of Bax,Beclin1 and LC3 Ⅱ in high-frequency ultrasound microbubbles group were higher( P < 0. 05),the number of green dots was more,and the protein expression levels of Bcl-2 and LC3 were lower( P < 0. 05). Conclusion Low-frequency ultrasound microbubbles can enhance the apoptosis and autophagy induced by paclitaxel in triple negative breast cancer cells by up-regulating the expression of Bax,Beclin1,LC3 Ⅱ protein and down-regulating the expression of Bcl-2 and LC3Ⅰ protein.

关 键 词：低频超声微泡 紫杉醇 三阴性乳腺癌 凋亡 自噬 

分 类 号：R737.9[医药卫生—肿瘤]