双硫仑联合铜通过调控视神经萎缩蛋白1诱导舌癌细胞凋亡  

Disulfiram combination with cooper induces apoptosis of tongue carcinoma cells by regulating OPA1 expression

作　　者：金杯 戴盼盼 孙旺 顾卫燕 林海升 施更生 JIN Bei;DAI Panpan;SUN Wang;GU Weiyan;LIN Haisheng;SHI Gengsheng(Department of Stomatology,Taizhou Hospital,Wenzhou Medical University,Taizhou 317000,China)

机构地区：[1]温州医科大学附属台州医院口腔科,浙江台州317000

出　　处：《口腔医学》2021年第4期295-298,317,共5页Stomatology

基　　金：台州市科技计划项目(XM20190604);恩泽医疗中心集团科研项目(18EZB11)。

摘　　要：目的探讨视神经萎缩蛋白1(OPA1)在双硫仑联合铜(DSF-Cu)诱导舌癌细胞凋亡中的作用。方法用不同浓度的DSF-Cu干预舌癌细胞Cal-27,MTT法检测细胞增殖活性,Annexin V-FITC/PI染色结合流式细胞仪检测细胞凋亡,荧光素酶发光法检测ATP含量,Western blot法检测OPA1蛋白表达水平。结果DSF-Cu能抑制舌癌细胞的增殖活性,促进细胞凋亡的发生,减少细胞ATP含量,并呈浓度依赖性。Western blot结果示低浓度DSF-Cu对OPA1蛋白表达无明显影响,而中、高浓度的DSF-Cu显著抑制OPA1蛋白表达水平。结论DSF-Cu可诱导舌癌细胞凋亡,其机制可能是通过抑制OPA1表达。Objective To investigate the effect of disulfiram combination with cooper(DSF-Cu) on the apoptosis of Cal-27 cells and its relation with optic atrophy 1(OPA1). Methods The Cal-27 cells were intervened with various concentrations of DSF-Cu. Cell viability was evaluated by MTT assays. The apoptosis rate was tested by Annexin V-FITC/PI staining combined with flow cytometry. The content of ATP was tested by luciferase luminescene. The protein expression level of OPA1 was detected by Western blotting. Results As compared with control group, DSF-Cu inhibited the proliferation activity of tongue carcinoma cells. Flow cytometric analysis showed an increase in the incidence of apoptosis. The content of ATP was decreased significantly. Western blotting results showed the expression of OPA1 was markedly decreased in high and medium concentration group, but there was no significant change in low concentration group. Conclusion DSF-Cu can induce apoptosis of Cal-27 cells, which may be achieved by inhibiting the expression of OPA1.

关 键 词：双硫仑 舌癌细胞 OPA1 细胞凋亡 

分 类 号：R739.8[医药卫生—肿瘤]