敲减MCCC2对前列腺癌细胞系DU145增殖、迁移和凋亡的影响  

Effects of MCCC2 knockdown on proliferation,migration and apoptosis of DU145 prostate cancer cells

作　　者：陈雪[1,2] 黄泽豪 郑承剑[2] CHEN Xue;HUANG Zehao;ZHENG Chengjian(School of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;School of Pharmacy,Naval Medical University,Shanghai 200433,China)

机构地区：[1]福建中医药大学药学院,福建福州350122 [2]海军军医大学药学院,上海200433

出　　处：《药学实践杂志》2021年第3期215-220,共6页Journal of Pharmaceutical Practice

基　　金：国家自然科学基金面上项目(81473328)。

摘　　要：目的应用慢病毒载体shRNA对DU145细胞中的3-甲基巴豆酰辅酶A羧化酶β亚基编码基因(3-methylcrotonyl-coenzyme A carboxylase 2,MCCC2)进行敲减,探究MCCC2对前列腺癌细胞生物学功能改变的影响。方法研究分为3组进行,其中shNC为未敲减MCCC2的DU145细胞对照组,shMCCC2为敲减MCCC2的DU145细胞实验组,DU145为未作任何处理的空白组。运用Western blot和qPCR检测DU145细胞系慢病毒shRNA敲减MCCC2后的表达,CCK8法检测敲减MCCC2对DU145细胞增殖的影响;Transwell检测敲减MCCC2对DU145细胞迁移的影响;流式细胞术检测敲减MCCC2对DU145细胞凋亡的影响。结果前列腺癌细胞系DU145经慢病毒shRNA敲减MCCC2后,shMCCC2组的MCCC2表达水平明显低于shNC组(0.22±0.02对0.61±0.06,P<0.001),shMCCC2组增殖(2.24±0.04对3.13±0.15)和迁移(23.96±1.85对49.73±0.63)均明显低于shNC组(P<0.001)、而shMCCC2组凋亡(12.64±0.30对3.68±0.02)明显高于shNC组(P<0.001)。结论敲减MCCC2可显著抑制DU145细胞的增殖和迁移并诱导细胞凋亡,提示MCCC2下调与DU145细胞系肿瘤生物学特性的改变具有一定相关性,有望成为前列腺癌的潜在治疗靶标。Objective To investigate the change of biological characteristics after stable knockdown of the coding gene of 3-methylcrotonyl-coenzyme A carboxylaseβsubunit(MCCC2)expression in DU145 by lentivirus shRNA.Methods Three groups were included in this study.shNC was the control group in which MCCC2 was negatively knocked down in DU145.shMCCC2 was the experimental group in which MCCC2 was knocked down.DU145 was the blank group without any treatment.The expression of MCCC2 was assessed by Western blot and qPCR.The proliferation of DU145 cells was detected by CCK8 assay.The migration ability of DU145 was detected by transwell.The apoptosis of DU145 cells was detected by flow cytometry.Results The expression level of MCCC2 in shMCCC2 group was significantly lower than that in shNC group(0.22±0.02 vs 0.61±0.06,P<0.001).The proliferation(2.24±0.04 vs 3.13±0.15)and migration(23.96±1.85 vs 49.73±0.63)of DU145 cells in shMCCC2 group was significantly lower than that in shNC group,whereas the apoptosis(12.64±0.30 vs 3.68±0.02)of DU145 cells in shMCCC2 was significantly higher than that in shNC group.Conclusion MCCC2 knockdown significantly inhibited the proliferation and migration,and induced apoptosis of DU145 cells,which indicated that the down-regulation of MCCC2 is correlated with the change of tumor biological characteristics of DU145 cell line and can be a potential target for the treatment of prostate cancer.

关 键 词：MCCC2 前列腺癌 增殖 迁移 凋亡 

分 类 号：R737.25[医药卫生—肿瘤]