bFGF对大鼠急性脊髓损伤组织内质网应激(ERS)信号通路相关蛋白CHOP、Caspase12表达的影响  

Effect of bFGF on the expression of proteins CHOP and Caspase12 related to endoplasmic reticulum stress(ERS)signaling pathway in acute spinal cord injury in rats

作　　者：葛小刚[1] 蔡旗旗[1] 牟财国 蔡杨阳 GE Xiaogang;CAI Qiqi;MOU Caiguo;CAI Yangyang(Department of Vascular Surgery,Taizhou First People′s Hospital in Zhejiang Province,Taizhou318020,China)

机构地区：[1]浙江省台州市第一人民医院血管外科,浙江台州318020

出　　处：《中国现代医生》2021年第11期36-39,F0003,共5页China Modern Doctor

基　　金：浙江省医药卫生科技计划项目(2020RC143);浙江省台州市科学技术局项目(1901ky64)。

摘　　要：目的探讨碱性成纤维细胞因子(bFGF)对大鼠急性脊髓损伤内质网应激(ERS)信号通路相关蛋白CHOP、Caspase12表达的影响。方法选取SD大鼠90只分为三组,每组各30只。Sham组为假手术组;SCI组采用HI-0400脊髓打击器制备急性脊髓损伤模型;bFGF组在SCI组基础上予bFGF蛛网膜下隙给药,评估损伤后不同时点后肢运动功能评分、脊髓细胞凋亡及脊髓组织CHOP、Caspase12表达情况。结果bFGF组损伤后21 d、28 d的BBB评分分别为(12.48±0.42)分、(13.53±0.47)分,均高于SCI组,差异有统计学意义(t=7.533、9.963,P<0.05)。bFGF组损伤后1 d、3 d、7 d细胞凋亡分别为(7.13±0.45)%、(12.42±1.21)%、(10.31±1.27)%,均低于SCI组,差异有统计学意义(t=4.684、8.945、6.507,P<0.05)。bFGF组CHOP及Caspase12表达分别为(7.66±0.74)分、(7.52±0.87)分,显著低于SCI组,差异有统计学意义(t=12.348、8.653,P<0.05)。结论bFGF可通过抑制ERS相关蛋白CHOP、Caspase12的表达,对大鼠急性脊髓损伤起保护作用。Objective To explore the effects of basic fibroblast growth factor(bFGF)on the expression of proteins CHOP and Caspase12 related to the endoplasmic reticulum stress(ERS)signaling pathway in acute spinal cord injury in rats.Methods A total of 90 SD rats were selected and divided into 3 groups,with 30 rats in each group.The sham group underwent the sham operation.The SCI group underwent the HI-0400 spinal cord percussion device to make acute spinal cord injury models.bFGF was administered to the subarachnoid space in the bFGF group on the basement of the SCI group.The hind legs motor function,spinal cord cell apoptosis and the expression of spinal cord tissue CHOP and Caspase12 at different times after injury were evaluated.Results The BBB scores of the bFGF group were(12.48±0.42)points and(13.53±0.47)points at 21 days and 28 days after injury,which were significantly higher than those in the SCI group,with statistical significance(t=7.533,9.963;P<0.05).The cell apoptosis in the bFGF group at 1 day,3 days,and 7 days after injury was(7.13±0.45)%,(12.42±1.21)%,(10.31±1.27)%,respectively,which were lower than those of the SCI group,the differences were statistically significant(t=4.684,8.945,6.507;P<0.05).The CHOP and Caspase12 expression in the bFGF group were(7.66±0.74)points and(7.52±0.87)points,respectively,which were significantly lower than those in the SCI group,with statistical significance(t=12.348,8.653;P<0.05).Conclusion bFGF can protect rats from acute spinal cord injury by inhibiting the expression of ERS-related proteins CHOP and Caspase12.

关 键 词：碱性成纤维细胞因子 内质网应激 大鼠急性脊髓损伤 保护作用 脊髓损伤评分 白细胞介素1-β转换酶家族12 

分 类 号：R363[医药卫生—病理学]