遵义地区肺结核合并糖尿病患者发生链霉素耐药的基因突变研究  

Streptomycin-resistant Gene Mutations in Patients with Tuberculosis and Diabetes in Zunyi

作　　者：周俊 唐竹 陶雪 韩晓静 陈云华 林牧[1] ZHOU Jun;TANG Zhu;TAO Xue;HAN Xiao-jing;CHEN Yun-hua;LIN Mu(Guizhou Aerospace Hospital,Zunyi 563000 China)

机构地区：[1]贵州航天医院,贵州遵义563000

出　　处：《内蒙古医学杂志》2021年第4期415-418,共4页Inner Mongolia Medical Journal

基　　金：贵州省遵义市科技局资助项目(编号:遵市科合支撑NS[019]6号,遵市科合社字(2018)30号)。

摘　　要：目的了解遵义地区肺结核合并糖尿病患者发生链霉素耐药的相关基因分子特征。方法通过TB-DNA、TR-RNA和抗酸染色(结果均为阳性)筛选出763例结核病患者,分为TB组和DM-TB组,经PCR-反向点杂交法检测耐药相关基因rpsL,对比分析两组基因突变特点;并搜集相关临床资料以探讨其与链霉素耐药发生的相关性。结果TB组的525例患者中有57例发生链霉素耐药,DM-TB组的207例患者中有24例发生链霉素耐药;DM-TB组发生链霉素耐药突变时,rpsL43位点突变率低于TB组,rpsL88位点突变率高于TB组,但差异不具统计学意义。结论遵义地区链霉素耐药患者突变位点以rpsL43N位点与rpsL43M突变为主。Objective To understand the molecular characteristics of genes related to streptomycin resistance in patients with tuberculosis and diabetes in Zunyi.Methods 763 patients with tuberculosis were screened by TB-DNA,TR-RNA,and acid-fast staining(all were positive).They were divided into TB group and DM-TB group.Compared and analyzed the resistance-related gene rpsL characteristics of gene mutations in the two groups by PCR-reverse dot hybridization.The clinical data were collected to explore its correlation with the occurrence of streptomycin r esistance.Results Streptomycin resistance occurred in 57 of the 525 patients in the TB group and 24 of 207 patients in the DM-TB group.The site mutation rate was lower than the TB group,and the rpsL88 site mutation rate was higher than the TB group,but the difference was not statistically significant.Conclusion The mutation sites of streptomycin resistance patients in Zunyi area were mainly rpsL43 N and rpsL43 M mutations.

关 键 词：肺结核 糖尿病 链霉素耐药 基因突变 

分 类 号：R521[医药卫生—内科学]