原发性胆汁性胆管炎患者PBMC中Caspase-1与IL-18水平观察  

Observation of caspase-1 and IL-18 levels in PBMC of patients with primary biliary cholangitis

作　　者：汪玉龙[1] 郝维敏[1] 郭志国 王晶[3] 刘志 WANG Yu-long;HAO Wei-min;GUO Zhi-guo;WANG Jing;LIU Zhi(Department of Clinical Laboratory,Suzhou Hospital Affiliated to Anhui Medical University,Suzhou 234000,China;Department of Digestive,Suzhou Hospital Affiliated to Anhui Medical University,Suzhou 234000,China;Department of Respiratory,Suzhou Hospital Affiliated to Anhui Medical University,Suzhou 234000,China)

机构地区：[1]安徽医科大学附属宿州医院检验科,宿州234000 [2]安徽医科大学附属宿州医院消化科,宿州234000 [3]安徽医科大学附属宿州医院呼吸科,宿州234000

出　　处：《肝脏》2021年第4期421-423,共3页Chinese Hepatology

基　　金：安徽省科技攻关项目(201904a07020060)。

摘　　要：目的研究原发性胆汁性胆管炎(PBC)患者外周血单个核细胞(PBMC)中天冬氨酸特异性半胱氨酸蛋白酶-1(Caspase-1)与白细胞介素-18(IL-18)的水平变化。方法选取安徽医科大学附属宿州医院2018年4月至2019年4月收治的54例PBC患者纳入研究对象,设为观察组,另外选取我院同期体检健康的志愿者54名纳入研究对象,设为健康组。患者(治疗前)与志愿者入院后抽取EDTA-K2抗凝全血,分离PBMC后行定量PCR扩增,检查Caspase-1、IL-18的mRNA水平。对比两组及不同肝硬化分级患者Caspase-1、IL-18水平差异,随后通过Spearman系数分析Caspase-1、IL-18水平与肝硬化分级的相关性。结果观察组Caspase-1、IL-18水平高于健康组(P<0.05)。不同患者Caspase-1、IL-18水平观察,C级>B级>A级,差异均有P<0.05。Caspase-1、IL-18水平与肝硬化分级呈正相关(均P<0.05)。结论PBC患者PBMC内Caspase-1、IL-18水平明显增高,且Caspase-1、IL-18水平表达与肝硬化分级呈正相关,Caspase-1、IL-18可能参与疾病的发生、发展过程。Objective To study the changes of aspartic acid-specific cysteine protease-1(Caspase-1)and interleukin-18(IL-18)levels in peripheral blood mononuclear cells(PBMC)of patients with primary biliary cholangitis(PBC).Methods A total of 54 PBC patients admitted to our hospital from April 2018 to April 2019 were selected as the subjects of the study and set as the observation group.In addition,54 volunteers who were physically healthy during the same period were selected as the subjects of the study and set as the healthy group.EDTA-K2 anticoagulant whole blood was extracted from patients(before treatment)and volunteers after admission.After PBMC separation,quantitative PCR amplification was performed to check mRNA levels of Caspase-1 and IL-18.The differences of Caspase-1 and IL-18 levels between the two groups and patients with different cirrhosis grades were compared.Then,Spearman coefficient was used to analyze the correlation between Caspase-1 and IL-18 levels and cirrhosis grades.Results The levels of Caspase-1 and IL-18 in the observation group were higher than those in the healthy group(P<0.05).The levels of Caspase-1 and il-18 in different patients were observed,and the levels of C-grade>B-grade>A-grade(all P<0.05).Caspase-1 and IL-18 levels were positively correlated with cirrhosis grade,both with P<0.05.Conclusion The levels of Caspase-1 and IL-18 in PBMC of PBC patients were significantly increased,and the expression levels of Caspase-1 and IL-18 were positively correlated with the grade of cirrhosis,and Caspase-1 and IL-18 could be involved in the occurrence and development of the disease.

关 键 词：原发性胆汁性胆管炎 外周血单个核细胞 天冬氨酸特异性半胱氨酸蛋白酶-1 白细胞介素-18 肝硬化分级 

分 类 号：R57[医药卫生—消化系统]