基于EST-SSR标记的油松种子园遗传多样性分析  

Genetic diversity analysis of Pinus tabulaeformis seed orchard based on EST-SSR marker

作　　者：代剑峰[1,2] 党磊 金雨晴[3] 周先清 贾海宽 张鸿景 DAI Jianfeng;DANG Lei;JIN Yuqing;ZHOU Xianqing;JIA Haikuan;ZHANG Hongjing(Hebei Academy of Forestry and Grassland Science,Shijiazhuang 050061,China;Hebei Technical Innovation Center of Improved Varieties of Forest Tree,Shijiazhuang 050061,China;Beijing Forestry University,Beijing 100083,China;Pingquan Qigou State Forest Farm,Chende 067500,China;Hulunbuir Honghua Erji Forestry Bureau National P.Sylvertris Fine Variety Base,Hulunbuir 021112,China)

机构地区：[1]河北省林业科学研究院,河北石家庄050061 [2]河北省林木良种技术创新中心,河北石家庄050061 [3]北京林业大学,北京100083 [4]平泉市国有七沟林场,河北承德067500 [5]呼伦贝尔市红花尔基林业局国家樟子松良种基地,内蒙古呼伦贝尔021112

出　　处：《河北农业大学学报》2021年第2期72-78,共7页Journal of Hebei Agricultural University

基　　金：河北省重点研发项目(20326333D);中央财政林业科技推广示范项目(冀TG[2014]014号);河北省省级省校科技合作开发资金支持项目(冀科政[2013]15号);林业公益性行业科研专项(201104022)。

摘　　要：以河北省平泉七沟油松(Pinus tabulaeformis)良种基地育种群体为研究对象,利用EST-SSR引物对来自4处种源的153个油松无性系进行了遗传多样性分析。在12个位点上共检查到62对等位基因,平均等位基因数为5.17;各位点的平均期望和观测杂合度(He和Ho)分别为0.414和0.403,近交系数(Fis)为0.018 6;4个种源群中大窝铺、东陵和七沟3个群体的平均有效等位基因数(Nea)、等位基因丰富度(AR)、观测杂合度(Ho)和期望杂合度(He)基本一致,都略高于宽城群体;平均群体差异系数(Fst)偏低,仅为0.015 6;种源群体间的差异系数(Pairwise Fst)极低,仅在0.009 9~0.071 7之间;NJ树图和主成分分析(PCA)的结果,显示各无性系的亲缘关系较为接近。综上,平泉油松种子园育种群体的遗传多样性水平较丰富,但是无性系之间的亲缘关系较近,群体结构不清晰。In this study the breeding population of Pinus tabulaeformis in Pingquan, Hebei Province was taken as the research object, the genetic diversity of 153 clones from 4 provenances was analyzed using EST-SSR primers. A total of 62 pairs of alleles were detected at 12 loci, and the mean allele number was 5.17. The mean expected and observed heterozygosity(He and Ho) of each point was 0.414 and 0.403 respectively, and the inbreeding coefficient(Fis) was 0.0186. The average number of effective alleles(Nea), allele abundance(AR), observed heterozygosity(Ho) and expected heterozygosity(He) of Dawopu, Dongling and Qigou populations in the4 provenance groups were basically the same, which were slightly higher than that of the Kuancheng population. The mean population difference coefficient(Fst) was low, only 0.0156.The coefficient of difference between provenance populations(Pairwise Fst) was also extremely low, ranging from 0.0099 to 0.0717. The results of NJ tree and principal component analysis(PCA) showed that all clones had close genetic relationships. In conclusion, the genetic diversity of the population in Pinus tabulaeformis variety orchard was abundant, but the clones were closely related and the population structure was unclear.

关 键 词：油松 种子园 EST-SSR 遗传多样性 群体结构 

分 类 号：S326[农业科学—作物遗传育种]