一株甲醇利用菌的分离、鉴定及特性研究  

Isolation,identification and growth characterization of a strain of methanol-utilizing bacteria

作　　者：张建云[1,2] 谷立坤 彭更[1] 史永胜 赵维文 崔方凯 臧亚奇 ZHANG Jianyun;GU Likun;PENG Geng;SHI Yongsheng;ZHAO Weiwen;CUI Fangkai;ZANG Yaqi(College of Environmental and Biological Engineering,Henan University of Engineering,Zhengzhou 451191,China;Henan Engineering Laboratory of Pollution Control and Coal Chemical Resources Comprehensive Utilization,Henan University of Engineering,Zhengzhou 451191,China)

机构地区：[1]河南工程学院环境与生物工程学院,河南郑州451191 [2]河南工程学院煤化工资源综合利用与污染治理河南省工程实验室,河南郑州451191

出　　处：《中国酿造》2021年第5期140-146,共7页China Brewing

基　　金：河南工程学院博士基金资助项目(D2010014);煤化工资源综合利用与污染治理河南省工程实验室开放基金资助项目(502002-B04)。

摘　　要：为了筛选高效利用甲醇的功能性微生物,从气化厂甲醇车间附近的土壤中分离筛选菌株,结合形态特征、生理生化特性和16S rDNA序列对其进行鉴定,并对菌株生长及甲醇降解条件进行研究。结果表明,分离得到一株细菌,编号为4311,经鉴定为甲基球形菌属(Methylopila sp.)。该菌株最适生长条件为初始甲醇体积分数1.0%,初始pH值5.0,氯化钠添加量0.05%,氮源为蛋白胨,其添加量为2 g/L。在此优化条件下,菌株4311对1.0%(V/V)甲醇的降解率达到85%。In order to screen the functional microorganisms that can efficiently utilize methanol,a strain of bacterium numbered 4311,was isolated and screened from the soil near the methanol workshop of a gasification plant.According to the results of morphological,biochemical and 16S rDNA sequence analysis,strain 4311 was identified as Methylopila sp.The optimal growth conditions of strain 4311 were initial methanol concentration 1.0%(V/V),initial pH 5.0,sodium chloride concentration 0.05%.The peptone was the optimal nitrogen source,and optimal peptone dosage was 2 g/L.Under these optimized conditions,the degradation rate of 1.0%(V/V)methanol by strain 4311 reached 85%.

关 键 词：甲醇利用菌 分离筛选 鉴定 生长条件 降解条件 

分 类 号：TQ223.121[化学工程—有机化工]