NO_(3)^(-)缓解小麦NH_(4)^(+)胁迫的生理机制研究  

Study on physiological mechanism of NO_(3)^(-) alleviating NH_(4)^(+) stress in wheat

作　　者：张燕[1,2] 张云秀[2] 吕雪梅 杜琬莹 冯波 李华伟[2] 王宗帅[2] 夏海勇[1,2] 樊守金 孔令安[1,2] ZHANG Yan;ZHANG Yunxiu;LÜXuemei;DU Wanying;FENG Bo;LI Huawei;WANG Zongshuai;XIA Haiyong;FAN Shoujin;KONG Ling’an(College of Life Science,Shandong Normal University,Jinan 250014,China;Crop Research Institute,Shandong Academy of Agricultural Sciences,Jinan 250100,China)

机构地区：[1]山东师范大学生命科学学院,济南250014 [2]山东省农业科学院作物研究所,济南250100

出　　处：《植物生理学报》2021年第2期480-492,共13页Plant Physiology Journal

基　　金：山东省自然科学基金(ZR2020MC087);山东省农业科技资金(园区产业提升工程)项目(2019YQ001);国家重点研发计划项目(2017YFD0300203和2018YFD0300601);山东省现代农业产业技术体系项目(SDAIT-01-06);国家现代农业产业技术体系项目(CARS-3-1-21)。

摘　　要：本实验研究了NO3-对单一NH4+胁迫下小麦(Triticum aestivum)根系生理特性的响应机制。结果表明:单一NH4+处理下,与对照相比,根系鲜重显著降低;吲哚乙酸(IAA)和茉莉酸含量显著降低,水杨酸含量显著升高;抗氧化酶活性显著降低,活性氧与丙二醛含量显著升高;氮代谢酶活性显著降低,谷氨酰胺含量显著增加。傅里叶转换红外光谱检测表明,纤维素、木质素等峰值增大,其含量也显著升高。非损伤微测结果表明,NH4+、H+外排速率以及IAA内流速率增加。NH4++NO3-处理能提高小麦根系IAA、茉莉酸等激素含量,降低水杨酸含量,提高抗氧化酶与氮代谢关键酶活性,降低NH4+、H+外排速率以及细胞壁结构性成分的含量,从而缓解NH4+抑制作用。综上所述,NH4++NO3-处理有效缓解了单一NH4+处理对小麦根系的抑制作用。In this study,we investigated the mechanism underlying which NO3-alleviates the NH4+stress upon root growth in wheat(Triticum aestivum).The results showed that the fresh weight of root was significantly reduced,the indoleacetic acid(IAA)and jasmonic acid contents were significantly decreased,and the salicylic acid content was significantly increased in roots of wheat plants under single NH4+treatment compared to the control.Meanwhile,the activities of antioxidant enzymes were significantly decreased and the contents of reactive oxygen species and malondialdehyde were significantly increased.The activities of nitrogen metabolism enzymes decreased and the content of glutamine were significantly increased.Fourier transform infrared determination showed that the peak values and contents of cellulose and lignin were significantly increased.Assay using non-invasive micro-test technology showed that the rates of NH4+and H+efflux and IAA influx were increased.NH4++NO3-treatment increased the contents of IAA,jasmonic acid and salicylic acid,improved the activities of antioxidant enzymes and key nitrogen metabolism enzymes,reduced the rate of NH4+and H+efflux,and decreased the contents of structural components in the cell wall,so as to alleviate the inhibitory effect of NH4+.In conclusion,NH4++NO3-treatment effectively alleviated the inhibitory effect of single NH4+treatment on the root growth in wheat.

关 键 词：单一NH_(4)^(+)胁迫 氮代谢酶 激素 抗氧化酶 NO_(3)^(-) 小麦 

分 类 号：S512.1[农业科学—作物学]