龙井茶多酚提取物对C57BL/6J小鼠肝脏氧化应激和代谢酶的影响  

Effects of Longjing tea polyphenol extracts on oxidative stress and metabolic enzymes in C57BL/6J mouse liver

作　　者：吕乐[1,2] 莫小叶 庞美霞 孙海燕[2] LYU Le;MO Xiao-ye;PANG Mei-xia;SUN Hai-yan(Postdoctoral Innovation Practice Base,Shenzhen Polytechnic;Shenzhen Key Laboratory of Fermentation,Purification and Analysis,Shenzhen Polytechnic,Shenzhen 518055,China)

机构地区：[1]深圳职业技术学院博士后创新基地 [2]深圳职业技术学院深圳市发酵精制检测系统重点实验室,深圳518055

出　　处：《天然产物研究与开发》2021年第5期743-749,790,共8页Natural Product Research and Development

基　　金：2020年广东省教育厅特色创新项目(2020KTSCX295);深圳市科技计划(JCYJ20170818114529521);深圳职业技术学院博士后启动基金(6019330004K,6019330003K)。

摘　　要：探究龙井茶多酚提取物对C57BL/6J小鼠肝脏氧化应激水平的影响,及其对小鼠肝脏内代谢酶系和转运蛋白的作用。灌胃给予C57BL/6J小鼠三个剂量龙井茶多酚提取物,采用病理切片、血清中谷草转氨酶(alanine aminotransferase,ALT)和谷丙转氨酶(aspartate aminotransferase,AST)含量来评价龙井茶多酚提取物对小鼠肝脏的损伤情况,通过测定小鼠肝脏谷胱甘肽(glutathione,GSH)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)来评价其对小鼠肝脏内氧化应激水平的影响,Western blotting分析其对小鼠肝脏中代谢酶系的影响。结果显示龙井茶多酚提取物可以通过增加肝脏中GSH-Px和CAT的含量来增强肝脏的氧化防御;通过诱导细胞色素450酶系2E1/3A11(cytochrome P4502E1/3A1,CYP2E1/3A11),硫酸转移酶1A1(sulfate transferase 1A1,SULT1A1),葡萄糖醛酸转移酶1A6(UDP-glucuronosyltransferases1A6,UGT1A6),多药耐药相关蛋白家族2(multi-drug resistance associated protein 2,MRP2)的蛋白表达,抑制P-糖蛋白(P-glycoprotein,P-gp)的蛋白表达来调节代谢。To investigate the effect of Longjing tea polyphenol extracts on the oxidative stress,the metabolic enzymes and transporters in C57BL/6J mouse liver.C57BL/6J mice were administrated with three doses Longjing tea polyphenol extracts by gavage.Liver injury was evaluated by pathological sections and the content of ALT and AST in serum.Liver GSH,GSH-Px,SOD and CAT were used to evaluate oxidative stress in mice livers.The relative protein expression level of CYP2E1/1A2/3A11,SULT1A1,UGT1A6,MRP2 and P-gp was evaluated by Western blotting.Results showed that Longjing tea polyphenol extracts could increase the content of GSH-Px and CAT in mice livers to enhance antioxidant defense.And it could induce the protein expression of CYP2E1/3A11,SULT1A1,UGT1A6,MRP2 and inhibit the protein expression of P-gp to adjust metabolism.

关 键 词：龙井茶多酚 氧化应激 CYP450 代谢酶 

分 类 号：R96[医药卫生—药理学]