Modulation of nitrate-induced phosphate response by the MYB transcription factor RLI1/HINGE1 in the nucleus  

作　　者：Zhihua Zhang Zhao Li Wei Wang Zhimin Jiang Liping Guo Xiaohan Wang Yangwen Qian Xiahe Huang Yongqiang Liu Xiujie Liu Yahong Liu Aifu Li Yu Yan Junpeng Xie Shouyun Cao Stanislav Kopriva Legong Li Fanjiang Kong Baohui Liu Yingchun Wang Bin Hu Chengcai Chu 

机构地区：[1]State Key Laboratory of Plant Genomics,Institute of Genetics and Developmental Biology,the Innovative Academy for Seed Design,Chinese Academy of Sciences,Beijing,China [2]School of Life Sciences,Guangzhou University.Guangzhou,China [3]College of Plant Science,Jilin University,Changchun,China [4]Biogle Genome Editing Center,Changzhou,China [5]State Key Laboratory of Molecular Developmental Biology,Institute of Genetics and Developmental Biology,the Innovative Academy for Seed Design,Chinese Academy of Sciences,Beijing,China [6]College of Advanced Agricultural Science,University of Chinese Academy of Sciences,Beijing,China [7]Botanical Institute,Cluster of Excellence on Plant Sciences,University of Cologne,Cologne,Germany [8]College of Life Sciences,Capital Normal University,Beijing,China

出　　处：《Molecular Plant》2021年第3期517-529,共13页分子植物（英文版）

基　　金：This work was supported by the National Key Research and Development Program of China(2016YFD0101801,2009CB118506);the National Natural Sciences Foundation of China(31771348,32002119);China Postdoctoral Science Foundation(2020M672569).

摘　　要：The coordinated utilization of nitrogen(N)and phosphorus(P)is vital for plants to maintain nutrient balance and achieve optimal growth.Previously,we revealed a mechanism by which nitrate induces genes for phosphate utilization;this mechanism depends on NRT1.1B-facilitated degradation of cytoplasmic SPX4,which in turn promotes cytoplasmic-nuclear shuttling of PHR2,the central transcription factor of phosphate signaling,and triggers the nitrate-induced phosphate response(NIPR)and N-P coordinated utilization in rice.In this study,we unveiled a fine-tuning mechanism of NIPR in the nucleus regulated by Highly Induced by Nitrate Gene 1(HINGE1,also known as RLI1),a MYB-transcription factor closely related to PHR2.RLI1/HINGE1,which is transcriptionally activated by PHR2 under nitrate induction,can directly activate the expression of phosphate starvation-induced genes.More importantly,RLI1/HINGE1 competes with PHR2 for binding to its repressor proteins in the nucleus(SPX proteins),and consequently releases PHR2 to further enhance phosphate response.Therefore,RLI1/HINGE1 amplifies the phosphate response in the nucleus downstream of the cytoplasmic SPX4-PHR2 cascade,thereby enabling fine-tuning of N-P balance when nitrate supply is sufficient.

关 键 词：RICE NIPR RLI1/HINGE1 PHR2 SPXs NUCLEUS 

分 类 号：Q94[生物学—植物学]