干旱区1株新欧文氏属菌的分离及溶磷特性  

Isolation and Phosphate-solubilizing Characteristics of a Novel Erwinia sp.Bacterium in Arid Environment

作　　者：侯娇 李莎[1] HOU Jiao;LI Sha(City College of Science and Technology,Chongqing University,Chongqing 402160,China)

机构地区：[1]重庆大学城市科技学院,重庆402160

出　　处：《环境科学与技术》2021年第2期19-24,共6页Environmental Science & Technology

基　　金：国家林业公益性行业科研专项(201404204);林业科技创新平台项目(2019132021)。

摘　　要：为开发适于使用的微生物磷肥,该研究从库布齐沙漠根际土中筛选了1株高效溶磷细菌,命名为GY35。根据菌株的16S rRNA基因分析和生理生化特征,将菌株GY35鉴定为欧文氏属(Erwinia)细菌。研究表明,菌株GY35的溶磷能力与其产生葡萄糖酸导致pH降低呈正相关。在NBRIP培养基中按1%的量接种GY35,24 h后培养基中的葡萄糖酸浓度升高到33.5 mg/L、pH降低至3.5,可溶性磷浓度达到363.9 mg/L,菌体数量达1.0×10^(9)CFU/mL。菌株GY35溶磷的最适温度为30℃,最适pH值为6.5~7.0,在碱性条件下的溶磷效率高于酸性条件。接种菌株GY35后,土壤中速效磷含量提高了15.1%,植物产量提高了30.7%。In order to develop suitable microbial phosphate fertilizer,a high efficient phosphate-solubilizing bacterium named GY35 was screened from the rhizosphere soil of Kubuqi desert.According to the 16 S r RNA gene analysis and the physiological and biochemical characteristics,the strain GY35 was identified as Erwinia sp.bacterium.Studies showed that the phosphate solubilizing ability of strain GY35 was positively correlated with the pH reduction caused by D-gluconic acid production.When GY35 was inoculated in the NBRIP medium at the concentration of 1%,the concentration of D-gluconic acid increased to 33.5 mg/L,p H decreased to 3.5,the concentration of soluble phosphorus reached 363.9 mg/L,and the number of mycelium reached 1.0×10^(9) CFU/mL after 24 hours.The optimal temperature and p H value of the strain GY35 were30℃ and 6.5~7.0 respectively.The phosphorus dissolving efficiency of strain GY35 was higher in alkaline condition than in acid condition.After inoculation with strain GY35,the content of available phosphorus in soil increased by 15.1%,and the plant yield increased by 30.7%.

关 键 词：溶磷菌 欧文氏菌 溶磷特性 土壤养分 

分 类 号：X172[环境科学与工程—环境科学] S154.39[农业科学—土壤学]