吸收放散试验与实时荧光定量PCR在ABO血型鉴定中的性能评价  

Absorption and elution test and real time PCR in ABO blood group identification: A comparative study

作　　者：李晓菲 苗天红[1] 孙崇云 刘婷婷 LI Xi-aofei;MIAO Tianhong;SUN Chongyun;LIU Tingting(Beijing Red Cross Blood Center,Bering 100088,China)

机构地区：[1]北京市红十字血液中心,北京100088

出　　处：《中国输血杂志》2021年第4期408-411,共4页Chinese Journal of Blood Transfusion

摘　　要：目的对吸收放散试验与实时荧光定量PCR应用于ABO血型鉴定的性能进行评估。方法筛选血清学格局表现为抗体减弱的血液标本,分别进行吸收放散试验、实时荧光定量PCR试验以及基因测序分析。其中以基因测序结果作为金标准,分别对吸收放散试验和实时荧光定量PCR试验进行一致性评价以及灵敏度、特异性比较。结果与测序结果(金标准)相比,吸收放散试验仅具有中度一致性,实时荧光定量PCR则具有很好的一致性。通过比较吸收放散试验和实时荧光定量PCR试验这2种方法的灵敏度和特异性,结果表明均具有统计学差异。结论在ABO疑难血型鉴定中,针对血清学格局表现为抗体减弱现象的标本,实时荧光定量PCR试验与测序结果符合度很好,检出弱抗原的能力强,误判率低,相比吸收放散试验,更有利于血型的准确定型。Objective To evaluate the performance of absorption and elution test and real time PCR in identifying ABO blood group. Methods The blood samples with weakened antibody expression serologically were screened and analyzed by absorption and elution test, real time PCR and gene sequencing. The gene sequencing results were used as the gold standard to assess the consistency of the other two methods, and the sensitivity and specificity were compared. Results Compared with the sequencing results(gold standard), absorption and elution test showed moderate consistency, while real time PCR showed good consistency. Both the sensitivity and specificity of absorption and elution test and real time PCR were statistically different.Conclusion There was a good agreement between real time PCR and gene sequencing in identifying suspicious ABO blood group with weakened antibody expression serologically. Real time PCR, with a low misjudgment rate, has strong ability to detect weak antigen and is more conducive to identify blood type compared with absorption and elution test.

关 键 词：ABO血型 吸收放散 实时荧光定量PCR 基因测序 

分 类 号：R457.11[医药卫生—治疗学]