补骨脂素对HTB-47和CRL-1932肾癌细胞系的增殖、侵袭和迁移的影响及相关机制  

Effects of psoralen on the proliferation,invasion and migration of HTB-47 and CRL-1932 renal cancer cell lines and related mechanisms

作　　者：袁令兴 李秀峰 顾传兰 卢世军 娄庆艳 刘源 林繁录 徐英民 Yuan Lingxing;Li Xiufeng;Gu Chuanlan;Lu Shijun;Lou Qingyan;Liu Yuan;Lin Fanlu;Xu Yingmin(Department of Urology,Linyi Central Hospital,Linyi 276400,China)

机构地区：[1]临沂市中心医院泌尿外科,276400

出　　处：《国际生物医学工程杂志》2021年第1期34-38,共5页International Journal of Biomedical Engineering

摘　　要：目的研究补骨脂素对体外培养的HTB-47和CRL-1932肾癌细胞的增殖、侵袭和迁移的影响,进一步探讨补骨脂素抑制肾癌的内在机制。方法实验组为含有30μg/ml补骨脂素的二甲基亚砜溶液处理的HTB-47和CRL-1932肾癌细胞,对照组为二甲基亚砜处理的肾癌细胞。用划痕实验、CCK8、Transwell、蛋白印迹法检测补骨脂素对肾癌细胞的影响。结果相比于对照组,实验组中补骨脂素处理的肾癌细胞的增殖、侵袭和迁移能力均明显受到抑制。补骨脂素处理的肾癌细胞中,细胞增殖抗原(MKI67)、增值细胞核抗原(PCNA)、基质金属蛋白酶2(MMP2)和MMP9的蛋白表达水平明显下降(均P<0.05)。结论补骨脂素在体外能明显抑制HTB-47和CRL-1932肾癌细胞的增殖、侵袭和迁移,其机制可能是通过调节MKI67、PCNA、MMP2和MMP9来抑制肾癌的进展。Objective To study the effect of psoralen on the proliferation,invasion and migration of HTB-47 and CRL-1932 renal cancer cells cultured in vitro,and to further explore the internal mechanism of psoralen inhibiting renal cancer.Methods The experimental group was HTB-47 and CRL-1932 renal cancer cells treated with dimethyl sulfoxide solution containing 30μg/ml psoralen,and the control group was renal cancer cells treated with dimethyl sulfoxide.Scratch test,CCK8,Transwell,and Western blot were used to detect the effect of psoralen on renal cancer cells.Results Compared with the control group,the proliferation,invasion and migration of renal cancer cells treated with psoralen in the experimental group were significantly inhibited.In the renal cancer cells treated with psoralen,the protein expression levels of MKI67,PCNA,MMP2 and MMP9 were significantly decreased(all P<0.05).Conclusions Psoralen can significantly inhibit the proliferation,invasion and migration of HTB-47 and CRL-1932 renal cancer cells in vitro.The mechanism may be to inhibit the progression of renal cancer by regulating MKI67,PCNA,MMP2 and MMP9.

关 键 词：补骨脂素 肾癌 HTB-47 CRL-1932 增殖 侵袭 

分 类 号：R737.11[医药卫生—肿瘤]