基于溴甲酚绿酸性染料比色的玛咖酒中总生物碱的检测方法构建  

Determination of Total Alkaloids in Maca Liquor by Colorimetry with Bromocresol Green Acid Dye

作　　者：王丹 郑若欣 马康 陈涛 吉喆 吴卫宇 李芳芳 兰余 曹晓念 袁强 张琴 刘小刚[1,3] WANG Dan;ZHENG Ruoxin;MA Kang;CHEN Tao;JI Zhe;WU Weiyu;LI Fangfang;LAN Yu;CAO Xiaonian;YUAN Qiang;ZHANG Qin;LIU Xiaogang(Luzhou Laojiao Co.Ltd.,Luzhou,Sichuan 646000;Sichuan Quality Inspection Co.Ltd.,Luzhou,Sichuan 646000;Luzhou Laojiao Health Liquor Co.Ltd.,Luzhou,Sichuan 646000,China)

机构地区：[1]泸州老窖股份有限公司,四川泸州646000 [2]四川国检检测有限责任公司,四川泸州646000 [3]泸州老窖养生酒业有限责任公司,四川泸州646000

出　　处：《酿酒科技》2021年第5期101-103,111,共4页Liquor-Making Science & Technology

摘　　要：建立玛咖酒中总生物碱含量的测定方法。采用分光光度法,以苦参碱为对照品,0.5%盐酸溶解,正丁醇-三氯甲烷提取,以溴甲酚绿为显色剂,在416 nm波长下进行测定。结果表明,苦参碱检测浓度在0~0.18 mg范围内与吸光度呈良好线性关系(R2=0.9954),正确度偏差范围在-8%~6%之间,方法精密度RSD=3.3%,加标浓度为5 mg/L和10 mg/L时回收率在90%~96%之间,回收率稳定在同一水平。In this study we established a method for the determination of total alkaloids in maca liquor.With matrine as the reference substance,0.5%HCl as the solution,n-butanol-chloroform as the extraction solution,and bromocresol green as the chromogenic reagent,alkaloids was determined by spectrophotometry at the wavelength of 416 nm.The results showed that the concentration of matrine had good linear relationship with the absorbance in the range of 0 to 0.18 mg(R2=0.9954),the accuracy was in the range of-8%to 6%,the RSD was 3.3%,the recovery rate was in the range of 90%to 96%when the spike concentration was 5 mg/L or 10 mg/L,and the recovery rate was stable.

关 键 词：玛咖酒 总生物碱 线性关系 正确度 精密度 回收率 

分 类 号：TS262.8[轻工技术与工程—发酵工程] TS261.7[轻工技术与工程—食品科学与工程]