Zichong granules(资冲颗粒)promote differentiation of ovarian granulosa-like cells from human embryonic stem cells in vitro  

作　　者：YAO Zouying WANG Jing LU Hua LI Limin ZHUO Tianxiu WAN Qian YIE Shangmian 

机构地区：[1]Department of Gynecology and Obstetrics,The Second Medical College/Teaching Hospital,Chengdu University of Traditional Chinese Medicine,Chengdu 610041,China [2]Gynecological Department of Traditional Chinese Medicine,Lishui Central Hospital,Lishui 323000,China [3]Department of Gynecology and Obstetrics,The Affinity Hospital,Shaanxi University of Chinese Medicine,Xian 712046,China [4]Department of Gynecology and Obstetrics,Affiliated Hospital,Chengdu University of Traditional Chinese Medicine,Chengdu 610072,China [5]Sichuan Academy of Traditional Chinese Medicine,Chengdu 610041,China

出　　处：《Journal of Traditional Chinese Medicine》2021年第2期203-211,共9页中医杂志（英文版）

基　　金：Supported by The National Basic Research Program of China:Study on the theory of"kidney governs reproduction"from the perspective of infertility(973 Plan,Grant Nos.2010CB530403 and 2010CB530400)。

摘　　要：OBJECTIVE:To investigate whether Zichong granules(资冲颗粒,ZCKL),a very effective herbal formula for treating infertility,have an impact on the differentiation of ovarian granulosa cells from human embryonic stem cells(hESCs)in vitro,and to explore the cellular mechanisms of its clinical effects.METHODS:Serum from ZCKL-medicated rats was prepared and used to treat mesoderm cells derived from hESCs for 6 d.Normal rat serum and a set of growth factors were used as negative and positive controls,respectively.RESULTS:ZCKL-medicated rat serum,but not normal rat serum,induced hESCs-derived mesoderm cells to differentiate into functional ovarian granulosa-like cells(OGLCs)in a similar manner to defined growth factors.The induced OGLCs resembled the morphology of native human granulosa cells,expressed granulosa cell-specific markers at both the mRNA and protein levels,produced high levels of estradiol and strongly responded to follicle-stimulating hormone stimulation.Furthermore,mRNA levels of follistatin,mothers against decapentaplegic homolog 8 and bone morphogenetic protein 6 were dynamically changed during the process.CONCLUSION:In the ZCKL treatment of infertility,one mechanism by which ZCKL may act is by influencing ovarian granulosa cell differentiation and development,possibly through the follistatin and BMP/SMAD signaling pathways.

关 键 词：human embryonic stem cells INFERTILITY serum MESODERM OVARIAN granulosa cells Zichong granules 

分 类 号：R285[医药卫生—中药学]