粉防己碱对急性髓系白血病细胞株KG-1增殖与凋亡的影响  

Effects of tetrandrine on the proliferation and apoptosis of KG-1 cells

作　　者：韩杰 孙艳丽[2] 王海英[3] 王爱红[3] 杨勇 王逸舒 胡振波[1] HAN Jie;SUN Yan-li;WANG Hai-ying;WANG Ai-hong;YANG Yong;WANG Yi-shu;HU Zhen-bo(Stem Cell and Regenerative Medicine Laboratory,Affiliated Hospital of Weifang Medical College,Weifang,Shandong 261042;Department of Medical Laboratory,Weifang Medical College,Weifang,Shandong 261053;Department of Hematology,Affiliated Hospital of Weifang Medical College,Weifang,Shandong 261042,China)

机构地区：[1]潍坊医学院附属医院干细胞与再生医学实验室,山东潍坊261042 [2]潍坊医学院医学检验学院,山东潍坊261053 [3]潍坊医学院附属医院血液科,山东潍坊261042

出　　处：《热带医学杂志》2021年第3期255-260,F0002,共7页Journal of Tropical Medicine

基　　金：国家自然科学基金面上项目(81570157)。

摘　　要：目的研究粉防己碱(Tet)对急性髓系白血病(AML)细胞株KG-1的作用及其机制。方法将KG-1细胞分为空白对照组、Tet 3.0μmol/L组、Tet 4.0μmol/L组、Tet 7.5μmol/L组、Tet 10.0μmol/L组、Tet 30.0μmol/L组,用CCK8法观察各组细胞的增殖活性;将KG-1细胞分为空白对照组、Tet 0.25μmol/L组、Tet 0.5μmol/L组、Tet 1.0μmol/L组、Tet 2.0μmol/L组,流式细胞术检测各组细胞的凋亡状况;将KG-1细胞分为空白对照组、Tet 0.25μmol/L、Tet 0.5μmol/L组、Tet 0.75μmol/L组、Tet 1.0μmol/L组,流式细胞术检测各组细胞的周期状况;将KG-1细胞分为空白对照组、Tet 0.25μmol/L组、Tet 0.5μmol/L组,并以1.0μmol/L全反式维甲酸分化诱导剂作为阳性对照组,流式细胞术检测各组细胞分化抗原CD11b、CD14的表达,瑞氏染色法观察各组细胞形态学的变化。结果CCK8检测结果显示,Tet 3.0μmol/L组、Tet 4.0μmol/L组、Tet 7.5μmol/L组、Tet 10.0μmol/L组、Tet 30.0μmol/L组细胞A450较空白对照组降低,自药物浓度为10.0μmol/L起差异有统计学意义(P<0.05),且药物浓度越高,细胞抑制率越高。流式细胞检测结果显示,Tet 0.25μmol/L组、Tet 0.5μmol/L组、Tet 1.0μmol/L组、Tet 2.0μmol/L组细胞总凋亡率较空白对照组显著升高,差异有统计学意义(P<0.05);且随着药物浓度升高,晚期凋亡率所占比例显著增高,差异有统计学意义(P<0.05)。流式细胞检测结果显示,Tet 0.25μmol/L组、Tet 0.5μmol/L组、Tet 0.75μmol/L组、Tet 1.0μmol/L组细胞在S期占细胞周期的比例较空白对照组显著升高,差异有统计学意义(P<0.05);随着药物浓度的升高,S期占细胞周期的比例依次升高,差异有统计学意义(P<0.05)。流式细胞检测结果显示,Tet 0.25μmol/L组、Tet 0.5μmol/L组细胞的CD11b、CD14的表达量均高于空白对照组,差异有统计学意义(P<0.05);瑞氏染色结果显示,Tet 0.25μmol/L组、Tet 0.5μmol/L组细胞有明显的分化状态,与分化诱导剂具有类似的Objective To study the effect of tetrandrine(Tet)on proliferation and differentiation of acute myeloid leukemia(AML)cell line KG-1 and its mechanism.Methods KG-1 cells were divided into blank control group,Tet 3.0μmol/L group,Tet 4.0μmol/L group,Tet 7.5μmol/L group,Tet 10.0μmol/L group,and Tet 30.0μmol/L group.The CCK8 method was used to observe cell proliferation activity in each group.KG-1 cells were divided into blank control group,Tet0.25μmol/L group,Tet 0.5μmol/L group,Tet 1.0μmol/L group,Tet 2.0μmol/L group,flow cytometry was used to detect the apoptotic status of each group.KG-1 cells were divided into blank control group,Tet 0.25μmol/L group,Tet 0.5μmol/L group,Tet 0.75μmol/L group,Tet 1.0μmol/L group,flow cytometry was used to detect cell cycle status in each group.KG-1 cells were divided into blank control group,Tet 0.25μmol/L group,and Tet 0.5μmol/L group,1.0μmol/L all-trans retinoic acid differentiation inducer was as positive control,flow cytometry was used to detect the expression of cell differentiation antigens GD11 b and CD14 in each group,and Wright staining was used to observe the changes of cell morphology in each group.Results After incubation of KG-1 leukemia cell line with tetrandrine,the OD value of cell enzyme level was significantly lower in Tet 3.0μmol/L group,Tet 4.0μmol/L group,Tet 7.5μmol/L group,Tet 10.0μmol/L group,and Tet 30.0μmol/L group than that in the blank control group,and the difference was statistically significant from the drug concentration was 10.0μmol/L(P<0.05).The higher the drug concentration and the higher was the cell inhibition rate.The total cell apoptosis rate was significantly higher in Tet 0.25μmol/L group,Tet 0.5μmol/L group,Tet1.0μmol/L group,and Tet 2.0μmol/L group than that in the blank control group(P<0.05),and the proportion of late apoptosis rate was significantly increased with the drug concentration increased(P<0.05).The proportion of S-phase cells in the cell cycle was significantly higher in Tet 0.25μmol/L group,Tet 0.5μm

关 键 词：粉防己碱 急性髓系白血病 KG-1细胞 S期阻滞 凋亡 分化 

分 类 号：R733.7[医药卫生—肿瘤]