超临界CO_(2)萃取桃金娘果及其挥发性成分研究  

Study on Supercritical CO_(2) Extraction of Rhodomyrtus tomentosa Fruit and Its Volatile Components

作　　者：银慧慧 冀晓雯[3] 孟菲[1,2] 赵武 姜源明[1,2] 覃振华 刘伟[1,2] YIN Hui-hui;JI Xiao-wen;MENG Fei;ZHAO Wu;JIANG Yuan-ming;QIN Zhen-hua;LIU Wei(Guangxi Veterinary Research Institute,Nanning 530001,China;Guangxi Key Laboratory of Veterinary Biotechnology,Nanning 530001,China;Guangxi Botanical Garden of Medicinal Plants,Nanning 530023,China)

机构地区：[1]广西壮族自治区兽医研究所,南宁530001 [2]广西兽医生物技术重点实验室,南宁530001 [3]广西壮族自治区药用植物园,南宁530023

出　　处：《中国兽药杂志》2021年第5期52-59,共8页Chinese Journal of Veterinary Drug

基　　金：广西科技重大专项-畜禽重大病害生态综合防控技术创新示范(桂科AA17204057);广西基本科研业务费专项(桂科专项17-5,桂科专项19-5)。

摘　　要：为研究超临界CO_(2)萃取桃金娘果工艺条件及其挥发性成分的物质组成,实现桃金娘资源综合利用,考察了不同夹带剂、萃取温度、CO_(2)流量、萃取压力、萃取时间对桃金娘果提取得率的影响,通过单因素、正交试验,以桃金娘果萃取物得率为考察指标对超临界CO_(2)萃取工艺参数进行优化;采用气相色谱-质谱分析桃金娘果萃取物中的挥发性成分。结果显示,最佳萃取工艺条件为:萃取压力12 Mpa、萃取温度35℃、CO_(2)流量17.5 g/min、萃取时间为150 min。在优化的萃取条件下桃金娘果萃取物得率为10.52%。桃金娘果萃取物共分离鉴定出20种挥发性成分,占总量的74.56%,主要为酯类、酸类、烃类、醇类、醛类成分。此研究为超临界CO_(2)萃取桃金娘果中的活性成分提供基础数据,并为桃金娘的进一步开发利用提供参考。This study was to investigate the optimum extraction conditions of Rhodomyrtus tomentosa fruit by supercritical CO_(2) fluid extraction and the composition of its volatile components.The effects of different cosolvent,extraction temperature,CO_(2) flow rate,extraction pressure,extraction time on the yield of extraction were investigated,and the single factor test and orthogonal test were used to optimize the extraction of Rhodomyrtus tomentosa fruit by supercritical CO_(2) fluid.The extraction rate of Rhodomyrtus tomentosa fruit was the index.The volatile ingredients in Rhodomyrtus tomentosa fruit were analyzed by gas chromatography-mass spectrometry.As a result,the optimal operation conditions included extraction temperature 35℃,CO_(2) flow rate 17.5 g/min,extraction pressure 12 Mpa,extraction time 150 min,and the extraction rate was 10.52%.20 volatile compounds were isolated and identified from Rhodomyrtus tomentosa fruit,accounting for 74.56%of the total volatile compounds.Hydrocarbons,alcohols,aldehydes,acids and esters were the major components.Supercritical CO_(2)fluid is suitable for the extraction of Rhodomyrtus tomentosa fruit,which can provide a reference for its further development and utilization.

关 键 词：超临界CO_(2) 桃金娘果 挥发性成分 单因素 正交实验 

分 类 号：S859.2[农业科学—临床兽医学]