CK5/6、P63、TTF-1和napsinA在腺癌及鳞状细胞癌鉴别诊断中的应用  

Application of CK5/6,P63,TTF-1 and napsinA in differential diagnosis of adenocarcinoma and squamous cell carcinoma

作　　者：陈显文 罗爱华[1] 曾洲红[1] CHEN Xianwen;LUO Aihua;ZENG Zhouhong(Department of Pathology,Gaozhou People's Hospital,Guangdong,Gaozhou 525200,China)

机构地区：[1]广东省高州市人民医院病理科,广东高州525200

出　　处：《中国医药科学》2021年第10期191-194,共4页China Medicine And Pharmacy

基　　金：广东省茂名市科技计划项目(170516221702132)。

摘　　要：目的分析肺小活检中应用免疫组化检验对腺癌及鳞状细胞癌的鉴别诊断研究。方法选择2019年1月至2020年6月随机抽取我院收治的30例肺原发性鳞状细胞癌以及50例肺原发性腺癌作为本研究对象,选择免疫组化EnViSion两步法对患者CK5/6(细胞角蛋白5/6)、P63、TTF-1(甲状腺转录因子1)和napsinA表达进行检测,对其诊断阳性率进行分析。结果鳞状细胞癌CK5/6阳性表达比例为80%,P63阳性表达比例为100%,而TTF-1以及napsinA阳性表达0;腺癌细胞癌CK5/6阳性表达比例为10%,P63阳性表达比例为6%,TTF-1阳性表达为96%,napsinA阳性表达为100%,对比两种疾病4种标志物表达,差异有统计学意义(P<0.05)。结论对于非小细胞肺癌标本而言,单纯通过形态学无法进行诊断时,可联合检验CK5/6、P63、TTF-1和napsinA,将诊断正确率提升。Objective To analyze the differential diagnosis of adenocarcinoma and squamous cell carcinoma(SCC)by immunohistochemical technique in small pulmonary biopsy.Methods 30 cases with primary pulmonary SCC and 50 cases with primary pulmonary adenocarcinoma(PPA)admitted to our hospital from January 2019 to June 2020 were randomly selected as the research objects.Immunohistochemical EnViSion two-step method was used to detect the expressions of cytokeratin 5/6(CK5/6),P63,thyroid transcription factor 1(TTF-1)and napsinA in patients,and the positive rates of four markers above were analyzed.Results The positive rate of CK5/6 and P63 in SCC was 80%and 100%,respectively,while the positive rate of TTF-1 and napsinA was 0.The positive rate of CK5/6,P63,TTF-1 and napsinA in adenocarcinoma was 10%,6%,96%and 100%,respectively.The expressions of four markers were significantly different between the two diseases,with statistically significant differences.The sensitivity and specificity of the four markers of SCC and adenocarcinoma were statistically significant between the groups.Conclusion For non-small cell lung cancer specimens,when the diagnosis cannot be made by morphology alone,CK5/6,P63,TTF-1 and napsinA can be used in combination to improve the diagnostic accuracy.

关 键 词：肺小活检 免疫组化 腺癌 鳞状细胞癌 鉴别诊断 

分 类 号：R734.2[医药卫生—肿瘤]