普通小麦–六倍体中间偃麦草易位系的抗条锈鉴定及应用评估  

Identification of seedling resistance to stripe rust in wheat–Thinopyrum intermedium translocation line and its potential application in breeding

作　　者：王音[1] 冯志威[3] 葛川 赵佳佳[2] 乔玲[2] 武棒棒 闫素仙 郑军 郑兴卫 WANG Yin;FENG Zhi-Wei;GE Chuan;ZHAO Jia-Jia;QIAO Ling;WU Bang-Bang;YAN Su-Xian;ZHENG Jun;ZHENG Xing-Wei(Department of Natural Sciences,Shanxi Normal University,Linfen 041000,Shanxi,China;Institute of Wheat Research,Shanxi Agricultural University,Linfen 041000,Shanxi,China;College of Agriculture,Shanxi Agricultural University/Shanxi Key Laboratory of Crop Genetics and Molecular Improvement,Taiyuan 030000,Shanxi,China)

机构地区：[1]山西师范大学临汾学院自然科学系,山西临汾041000 [2]山西农业大学小麦研究所,山西临汾041000 [3]山西农业大学农学院/作物遗传与分子改良山西省重点实验室,山西太原030000

出　　处：《作物学报》2021年第8期1511-1521,共11页Acta Agronomica Sinica

基　　金：中国博士后科学基金项目(2020M670701);山西省重点研发计划项目(201903D221074,201903D221075);山西省重点实验室项目(201705D111008-22)资助。

摘　　要：小麦近缘属种中含有丰富的抗病资源,前期从普通小麦与六倍体中间偃麦草的杂交、回交后代中,鉴定获得稳定的小麦易位系新种质ZH811。为发掘和利用该易位系的抗性基因,对ZH811进行苗期分小种的条锈病鉴定、田间农艺性状考察、分子细胞学特性和主要品质性状分析;利用随机扩增多态性DNA(Random Amplified Polymorphic DNA,RAPD)开发易位片段的特异标记。结果表明,ZH811苗期对条锈菌条中29、31、32、33、34以及水源4、5和7号的混合菌种具有较强的抗性,其抗性源于5D染色体短臂含有来源于Ee基因组的小片段易位;SCAR标记(Sequence Characterized Amplified Region,SCAR)和黑芒可作为易位片段追踪的分子和形态标记;ZH811的主要农艺性状接近当前黄淮北片麦区的主推品种,HMW-GS组合类型是“1,17+18,5+10”,3个位点均为优质亚基,各项品质指标达到中强筋的标准;易位片段具有增加穗粒数的效应,不含降低品质的连锁累赘。ZH811可为培育高产、抗病和优质小麦新品种提供重要的中间材料。There are abundant disease resistance resources in relative genus of wheat.The translocation line ZH811 derived from progeny of wheat and Th.intermedium hybrids,was selected for evaluation of stripe rust resistance.A series of stripe rust races such as CYR29,CYR31,CYR32,CYR33,CYR34,Suwon-4,Suwon-5,and Suwon-7 were used to record stripe responses at seedling stage.Agronomical traits,quality traits,and molecular cytogenetic analysis were also performed.Moreover,specific molecular markers located on alien segment was developed by RAPD method.The results indicated that ZH811 was highly resistant to all tested races at seedling stage.And it further proved that the resistance was conferred by a small-fragment-translocation from the Ee genome on 5DS chromosome.The SCAR markers and black awn traits could be used to trace the translocation fragment.ZH811 possessed similar agronomic traits with the commercial cultivars in the Yellow-Huaihe-Haihe Rivers region of wheat.The translocation fragment may be associated with the increase of grain number.The components of the Glu-1 were good-quality subunits,including 1,17+18,and 5+10,and each quality index met the standard of moderate gluten.The alien chromosomal fragment had no obvious linkage drag to grain quality performance.Based on these findings,ZH811 could be used as a potential material for wheat breeding in high yield,disease resistance,and high quality.

关 键 词：条锈 易位系 原位杂交 分子标记 

分 类 号：S512.1[农业科学—作物学]