枯草芽孢杆菌(Bacillus subtilis)BJ3-2发酵薏米高产川芎嗪和溶纤酶体系优化  

Optimization of high-yield tetramethylpyrazine and fibrinolytic enzyme of Bacillus subtilis BJ3-2-fermented adlay

作　　者：文安燕 秦礼康 曾海英[1] 朱怡 WEN Anyan;QIN Likang;ZENG Haiying;ZHU Yi(School of Liquor and Food Engineering,Guizhou University,Guiyang 550025,China;National&Local Joint Engineering Center for the Development and Utilization Technology of Drug and Food Resources in Southwest China,Guiyang 550025,China;Plant Protection and Plant Quarantine Station of Guizhou Province,Guiyang 550001,China)

机构地区：[1]贵州大学酿酒与食品工程学院,贵州贵阳550025 [2]贵州大学,西南药食两用资源开发利用国家地方联合工程研究中心,贵州贵阳550025 [3]贵州省植保站,贵州贵阳550001

出　　处：《食品与发酵工业》2021年第10期178-184,191,共8页Food and Fermentation Industries

基　　金：贵州省自然科学基金(黔科合基础1111号);贵州省科技厅重大专项(黔科合重大专项字6011号;黔科合重大专项字6010-5);贵州农业厅项目(黔财农〔2018〕81号)。

摘　　要：前期初探发现枯草芽孢杆菌(Bacillus subtilis)BJ3-2发酵精薏米可高产川芎嗪[2.97 mg/g干基(dry weight,DW)]和纤溶酶(720.84 U/g)。该研究以糙薏米、精薏米和碎薏米为原料,以B.subtilis BJ3-2为发酵菌株,通过单因素及Box-Behnken试验构建高产川芎嗪和溶纤酶的薏米发酵体系。结果表明,接种量为9.0%、发酵温度为40℃和发酵时间为93 h时,B.subtilis BJ3-2发酵糙薏米中川芎嗪产量为6.15 mg/g DW、溶纤酶酶活为2236.47 U/g,而以接种量为8.1%、发酵温度为38℃和发酵时间为96 h时,发酵精薏米中川芎嗪产量为6.94 mg/g DW、溶纤酶酶活为2142.18 U/g,但碎薏米中川芎嗪产量和溶纤酶酶活均明显偏低。此外,B.subtilis BJ3-2分别发酵的3种薏米,其川芎嗪含量均高于B.subtilis BJ3-2发酵黄豆和CICC 20637发酵薏米。因此,综合考虑加工成本,B.subtilis BJ3-2发酵糙薏米可作为高产川芎嗪和溶纤酶的最佳发酵体系。Our previous study found that high-yield tetramethylpyrazine(2.97 mg/g DW)and fibrinolytic enzyme(720.84 U/g)were obtained in Bacillus subtilis BJ3-2-fermented polished adlay.Taking dehulled,polished and broken adlay as raw materials and B.subtilis BJ3-2 as fermentation strain,the fermentation conditions for high-yield tetramethylpyrazine and fibrinolytic enzyme production were optimized by single factor and Box-Behnken experiments in this study.Results showed that the tetramethylpyrazine yield and fibrinolytic enzyme activity of B.subtilis BJ3-2-fermented dehulled adlay were 6.15 mg/g DW and 2236.47 U/g when inoculation amount,fermentation temperature and fermentation time were 9.0%,40℃and 93 h.The tetramethylpyrazine yield and fibrinolytic enzyme activity of B.subtilis BJ3-2-fermented polished adlay were 6.94 mg/g DW and 2142.18 U/g when inoculation amount,fermentation temperature and fermentation time were 8.1%,38℃and 96 h.The tetramethylpyrazine yield and fibrinolytic enzyme activity in B.subtilis BJ3-2-fermented broken adlay were low even after optimization.Additionally,the tetramethylpyrazine yield of B.subtilis BJ3-2-fermented adlay was higher than that of B.subtilis BJ3-2-fermented soybean and CICC 20637-fermented adlay.Therefore,considering the processing cost of adlay,B.subtilis-fermented dehulled adlay could be the optimal fermentation system to obtain high-yield tetramethylpyrazine and fibrinolytic enzyme.

关 键 词：薏米 枯草芽孢杆菌 川芎嗪 溶纤酶 Box-Behnken试验 

分 类 号：TS210.4[轻工技术与工程—粮食、油脂及植物蛋白工程]