2种拮抗云杉梢枯病细菌的抑菌活性研究  

Antibacterial Activity Study of Two Antagonistic Bacteria of Dothiorella gregaria

作　　者：曹娜 祝文博[1] 黄麒 刘雪峰[1] CAO Na;ZHU Wenbo;HUANG Qi;LIU Xuefeng(School of Forestry,Northeast Forestry University,Harbin 150040,China)

机构地区：[1]东北林业大学林学院,哈尔滨150040

出　　处：《森林工程》2021年第3期72-78,共7页Forest Engineering

摘　　要：用平板对峙法研究贝莱斯芽胞杆菌(Bacillus velezensis)和甲基营养型芽孢杆菌(B.methylotrophicus)对云杉梢枯病病原菌寄生小穴壳(Dothiorella gregaria)的抑菌活性,测定2种拮抗菌的生长曲线和不同培养时间、不同体积分数的发酵液抑菌活性及其稳定性,通过光学显微镜研究抑菌机理。结果表明,2种拮抗菌对云杉梢枯病病原菌均有较强的抑制作用,抑菌率分别为57.50%、56.67%。贝莱斯芽胞杆菌发酵液0~12 h对病原菌菌丝体抑菌率几乎为0;24 h抑菌率达到最大56.52%;24~72 h,抑菌率处于43.48%~47.83%。甲基营养型芽孢杆菌发酵液0~12 h对病原菌菌丝体抑菌率几乎为0;24 h抑菌率达到最大52.17%;24~60 h,抑菌率处于47.83%~52.17%;60~72 h,抑菌率有所下降,抑菌率最低为43.48%。2种拮抗菌发酵液的抑菌活性随着体积分数的升高而上升。2种无菌滤液对温度不敏感,经不同酸碱度处理后抑菌活性下降。通过显微镜观察,2种拮抗菌发酵液能使病原菌菌丝发生变粗彭大、表面粗糙等形态变化。The plate confrontation method was used to study the antibacterial activity of Bacillus velezensis and B.methylotrophicus against Dothiorella gregaria,and the grow curve of two kinds of antagonistic bacteria and the antibacterial activity of different incubation time and different concentration were measured.and antibacterial mechanism was studied by optical microscope.The results showed that two kinds of antagonistic bacteria had significant inhibition effect against D.gregaria with the antibacterial rate of 57.50%and 56.67%,respectively.The antibacterial rate of sterile culture filtrate of B.velezensis was nearly 0 from 0 to 12 h.The maximum antibacterial rate was 56.52%at 24 h,and the antibacterial rate was 43.48%-47.83%in 24-72 h.The antibacterial rate of sterile culture filtrate of B.methylotrophicus was nearly 0 from 0 to 12 h.The maximum antibacterial rate was 52.17%at 24 h,and the antibacterial rate was 47.83%-52.17%in 24-60 h.In 60-72 h,the antibacterial rate decreased,and the lowest was 43.48%.The antibacterial activity of two kinds of antagonistic bacteria sterile culture filtrate increased with the increase of the volume fraction.Two kinds of sterile culture filtrate was not sensitive to temperature and the antibacterial activity decreased after different pH treatment.Under the microscope,two kinds of antagonistic bacteria sterile culture filtrate can make hyphae coarsen,bent and enlarged.

关 键 词：贝莱斯芽胞杆菌 甲基营养型芽孢杆菌 青海云杉 云杉梢枯病 抑菌活性 

分 类 号：S763.15[农业科学—森林保护学]