SD大鼠常见自发性非肿瘤病变及其发生率  

Common spontaneous non-neoplastic lesions and their incidence in SD rats

作　　者：蔡婷峰[1] 邓莹玉[1] 陆丰荣[1] 陈丹苹 黄曼琪 马超亚[1] 宋向荣[1] 王海兰[1] CAI Ting-feng;DENG Ying-yu;LU Feng-rong;CHEN Dan-ping;HUANG Man-qi;MA Chao-ya;SONG Xiang-rong;WANG Hai-lan(Guangdong Province Hospital for Occupational Disease Prevention and Treatment,Guangdong Provincial Key Laboratory of Occupational Disease Prevention and Treatment,Guangzhou Guangdong,510300,China)

机构地区：[1]广东省职业病防治院,广东省职业病防治重点实验室,广东广州510300

出　　处：《职业与健康》2021年第4期468-471,475,共5页Occupation and Health

基　　金：广东省医学科学技术研究基金(A2018311)。

摘　　要：目的了解1 094只SD大鼠常见自发性非肿瘤病变及其发生率,为食品、保健品、药品、农药、化学品等毒理学安全性评价和科学研究提供基础数据。方法采用5~6周龄SPF级SD大鼠,1 094只,雌雄各半。检疫5天,常规饲料喂饲104周后处死,进行组织病理学检查。汇总1 094只慢性毒性实验对照组大鼠自发性非肿瘤病变发生情况并计算发生率。结果 SD大鼠的常见自发性非肿瘤病变中发生率最高为肝细胞脂肪变性(50.5%)、肾小管管型(32.7%)、脾色素沉着(23.7%)、脾脏髓外造血(22.9%)、心肌细胞坏死(13.5%)。大脑充血、小脑出血、肝枯否细胞增生、脾色素沉着、脾多核巨细胞增生/组织细胞肿胀、脾脏白髓萎缩、肾小管坏死及肾间质/肾小管矿化在雌性大鼠中的发生率较高,与雄性大鼠比较,差异均有统计学意义(均P<0.05);心肌细胞空泡变性、心脏炎症细胞浸润、肝细胞水肿、肝窦扩张、肺充血/淤血、肺间质矿化、肺色素沉着、肾间质炎细胞浸润、肾小管嗜碱性变性、慢性进行性肾病及肾囊肿在雄性大鼠中的发生率较高,与雌性大鼠比较,差异均有统计学意义(均P<0.05)。结论慢性毒性实验中SD大鼠常见自发性非肿瘤病变病变种类繁多,发生率不同,本次实验结果可以作为食品、保健品、药品、农药、化学品等毒理学安全性评价和科学研究的基础数据。Objective To summarize the spontaneous non-tumor lesions and their incidence in 1 094 SD rats,provide basic data for toxicological safety evaluation and scientific research of food,health products,drugs,pesticides,and chemicals. Methods Totally1 094 SPF SD rats aged 5-6 weeks were used,half male and half female. The animals were quarantined for five days,and were killed after 104 weeks of conventional feed,to perform the histopathological examination. The spontaneous non-tumor lesions in1 094 rats of chronic toxicity experiment control group was collected and the incidence rate was calculated. Results The common spontaneous non tumor lesions with the highest incidence in SD rats were hepatocyte steatosis(50.5%),renal tubular cast(32.7%),splenic pigmentation(23.7%),splenic extramedullary hematopoiesis(22.9%),myocardial cell necrosis(13.5%). The incidence of cerebral hyperemia,cerebellar hemorrhage,hyperplasia kupffer cell,splenic pigmentation,splenic multinucleated giant cell proliferation/histiocyte swelling,splenic white pulp atrophy,renal tubules necrosis/renal mineralization in female rats were higher than those in male rats(all P<0.05). The incidence of myocardium vacuolation,heart inflammatory cells infiltrate,hepatocyte hydropic degeneration,hepatic sinusdilation,liver yperemia/congestion,pulmonary interstitial mineralization,pulmonary pigmentation,renal interstitial inflammatory cell infiltration,renal tubular basophilic degeneration,chronic progressive nephropathy and renal cyst in male rats were higher than those in female rats,and the differences were statistically significant(all P<0.05). Conclusion In chronic toxicity experiment,there are many kinds of common spontaneous non tumor lesions in SD rats,and the incidence rate is different,which can provide basic data for toxicological safety evaluation and scientific research of food,health care products,drugs,pesticides,and chemicals.

关 键 词：SD大鼠 自发性 非肿瘤病变 常见 发生率 

分 类 号：R-332[医药卫生]