C57BL6近交系小鼠前列腺癌原位移植瘤模型建立及比较  

Establishment and comparison of orthotopic transplantation tumor model of prostate cancer in C57BL6 inbred line mice

作　　者：张政 张欣 付启航 岳悦 王雷雷 陈辉[4] 徐阳阳 ZHANG Zheng;ZHANG Xin;FU Qihang;YUE Yue;WANG Leilei;CHEN Hui;XU Yangyang(Department of Radiotherapy,Affiliated Tumor Hospital,Harbin Medical University,Harbin,Heilongjiang 150000,China;College of Chemistry and Environmental Engineering,Harbin University of Science and Technology,Harbin,Heilongjiang 150000,China;Department of Imaging,Affiliated Tumor Hospital,Harbin Medical University,Harbin,Heilongjiang 150000,China;Department of Urological Surgery,Affiliated Tumor Hospital,Harbin Medical University,Harbin,Heilongjiang 150000,China)

机构地区：[1]哈尔滨医科大学附属肿瘤医院放疗科,哈尔滨150000 [2]哈尔滨理工大学化学与环境工程学院,哈尔滨150000 [3]哈尔滨医科大学附属肿瘤医院,哈尔滨150000 [4]哈尔滨医科大学附属肿瘤医院泌尿外科,哈尔滨150000

出　　处：《重庆医学》2021年第10期1765-1768,1772,共5页Chongqing medicine

基　　金：黑龙江省自然科学基金面上项目(H2017046)。

摘　　要：目的探讨瘤丝注射法建立C57BL6前列腺癌原位移植瘤模型的应用价值,并与传统的前列腺原位癌成瘤方法进行比较。方法取36只7周龄(18~20 g)雄性C57BL6近交系小鼠,将其分成A、B、C 3组,每组12只,A组为瘤丝注射组、B组为细胞注射组、C组为肿块移植组。观察每组小鼠的成瘤情况、转移情况、周围组织浸润情况、小鼠生存期、操作难易程度等指标。结果A、B、C 3组小鼠成瘤率分别为100%、91.67%、91.67%,3组小鼠成瘤率比较差异无统计学意义(P>0.05)。A、B、C组小鼠肺转移率分别为83.33%、25.00%、33.33%,肝转移率分别为75.00%、25.00%、25.00%,A组小鼠的肺、肝转移率明显高于B、C组(P<0.05);B、C组小鼠的肺、肝转移率比较,差异无统计学意义(P>0.05)。3组小鼠均出现广泛的腹腔浸润。A、B、C组小鼠的生存时间分别为(17.33±2.19)、(14.7±2.39)、(13.58±2.43)d,A组小鼠的生存时间明显长于B、C组(P<0.05);B、C组小鼠的生存时间比较差异无统计学意义(P>0.05)。A组操作难度要求相对较低,B组成瘤方式易导致细胞液外溢造成人为肿瘤转移,对术者注射器持握的平稳性要求较高,C组需对前列腺筋膜进行缝合,要求术者具有较好的显微手术技术。结论相比于传统的成瘤方法,瘤丝注射法具有更高的成瘤率、转移率和更长的生存时间。Objective To investigate the application value of C57BL6 orthotopic tumor model of prostate cancer established by using the fibroblast injection method and to compare it with the traditional orthotopic tumor formation method of prostate cancer.Methods Thirty-six 7-week-old(18-20 g)male C57BL6 inbred mice were randomly divided into the group A,B and C,12 cases in each group.The group A was the fibroblast injection,the group B was the cell injection and the group C was the tumor transplantation.The tumorigenesis,metastasis,infiltration of surrounding tissues,survival time and operation difficulty were observed in the mice of each group.Results The tumorigenicity rates of the group A,B and C were 100%,91.67%and 91.67%respectively.The lung metastasis rates of the group A,B and C were 83.33%,25.00%and 33.33%,and the liver metastasis rates were 75.00%,25.00%and 25.00%respectively.The lung and liver metastasis rates in the group A were significantly higher than those in the group B and C(P<0.05);the lung and liver metastasis rates had no statistical difference between the group B and C(P>0.05).The three groups appeared the extensive abdominal infiltration.The survival time of the three groups was(17.33±2.19)d,(14.7±2.39)d and(13.58±2.43)d respectively,and the survival time of the group A was significantly longer than that of the group B and C(P<0.05).The survival time had no statistical difference between the group B and C(P>0.05).The operation difficulty requirement in the group A was relatively low,the tumor formation mode in the group B was easy to lead to the overflow of cell fluid and cause the artificial tumor metastasis,which required the high smoothness for the operator holding the syringe.The group C needed to suture the prostate fascia,which required the operator to have good microsurgical technique.Conclusion Compared with the traditional tumor formation method,the fibroblast injection method has the higher tumor formation rate,metastasis rate and longer survival time.

关 键 词：前列腺肿瘤 移植瘤模型 C57BL6近交系小鼠 病理 转移 

分 类 号：R737.25[医药卫生—肿瘤]