CRISPR-Cas9技术在肿瘤耐药基因筛选中的研究进展  

Application of CRISPR-Cas9 technology in the screening of tumor resistance genes: A review

作　　者：张洁[1] 杨谭[1] 卢永刚[1] ZHANG Jie;YANG Tan;LU Yong‑gang(Liuzhou Municipal Liutie Central Hospital,Liuzhou 545007,China)

机构地区：[1]广西壮族自治区柳州市柳铁中心医院药剂科,广西柳州545007

出　　处：《海南医学院学报》2021年第11期872-875,共4页Journal of Hainan Medical University

基　　金：柳州市科技计划项目(2019BJ10612)。

摘　　要：耐药是使用肿瘤分子靶向药物治疗肿瘤面临的主要问题,目前筛选耐药基因常用策略主要为功能性基因筛选。近年来,CRISPR-Cas9基因编辑技术因具有精准、简便、高效的特点,在肿瘤相关基因的功能学研究中被广泛应用。本文就CRISPR-Cas9文库筛选技术的原理和在功能性基因筛选方面的应用进行了综述,同时对该技术的应用前景进行了展望。Drug resistance is a major problem when using molecular targeted drugs for tumors.Currently,functional gene screening is the most common strategy for screening drug resistance genes.In recent years,CRISPR-Cas9 gene-editing technology has been widely used in functional studies on tumor-related genes because of its high accuracy,simplicity,and efficiency.In this paper,the principle of CRISPR-Cas9 library screening technology and its application in functional gene screening are reviewed.At the same time,the application prospect of the CRISPR-Cas9 technology is forecasted.

关 键 词：CRISPR-Cas9技术 功能基因 筛选 

分 类 号：R730.5[医药卫生—肿瘤]