KCNMA1在胰腺癌细胞系中的表达及其作用机制  

Expression of KCNMA1 in pancreatic cancer cell lines and action mechanism of expressed product

作　　者：严江渝 张策[1] YAN Jiang-yu;ZHANG Ce(Key Lalioratory of Cellular Physiology,Shanxi Medical University,Taiyuan 030001,Shcuixi Province,China)

机构地区：[1]山西医科大学细胞生理学教育部重点实验室,山西太原030001

出　　处：《中国生物制品学杂志》2021年第4期394-399,共6页Chinese Journal of Biologicals

摘　　要：目的在胰腺癌(pancreatic cancer,PC)细胞系中表达KCNMA1,并探讨其在PC细胞增殖、凋亡、迁移及侵袭中的作用。方法在PC细胞系Patu-8988T、MiaPaCa-2、PANC-1、Capan-1、AsPC-1、CFPAC-1及BxPC-3中培养KCNMA1,RT-PCR法检测表达情况。使用siRNA沉默PC细胞中KCNMA1基因,CCK-8法检测其对细胞增殖的影响;流式细胞术检测其对细胞凋亡的影响,Western blot法检测凋亡相关蛋白表达;Transwell检测其对细胞迁移及侵袭的影响。结果 KCNMA1在MiaPaCa-2和PANC-1细胞中的表达明显高于其他细胞系。与对照(NC)组相比,接种MiaPaCa-2和PANC-1细胞24 h后,各干扰组A450即明显升高(P<0.05);细胞凋亡比例升高至12.72%和25.1%,凋亡相关蛋白BAX、Cleaved-PARP1、Actived-Caspase-3表达增加;迁移细胞数分别减少至(103.8±10.6)和(117±12.2)个,侵袭细胞数分别减少至(63±5.8)和(116.8±9.8)个(P均<0.01)。结论 KCNMA1在PC细胞系MiaPaCa-2和PANC-1中表达较高,沉默KCNMA1基因能够有效抑制PC细胞的增殖、迁移及侵袭能力,促进PC细胞凋亡。Objective To express KCNMA1 in pancreatic cancer(PC) cell lines and investigate its role in the proliferation,apoptosis,migration and invasion of PC cells. Methods KCNMA1 was cultured in PC cell lines Patu-8988 T,MiaPaCa-2,PANC-1,Capan-1,AsPC-1,CFPAC-1 and BxPC-3 respectively,of which the expression was determined by RT-PCR. The KCNMA1 gene in PC cells was silenced by small interfering RNA(siRNA),while the cell proliferation level was determined by CCK-8 method,the apoptosis by flow cytometry,the expressions of apoptosis-related proteins by Western blot,and the migration and invasion by Transwell assay. Results The expression levels of KCNMA1 in MiaPaCa-2 and PANC-1 cells were significantly higher than those in other cell lines. Compared with that in normal control(NC) group,the A450 values of MiaPaCa-2 and PANC-1 cells 24 h after transfection with siRNA increased significantly(P< 0. 05),while the proportion of apoptotic cells increased to 12. 72% and 25. 1% respectively,the expressions of BAX,Cleaved-PARP1 and Actived-Caspase-3 protein increased,the numbers of migrated MiaPaCa-2 and PANC-1 cells decreased to 103. 8 ± 10. 6 and 117 ± 12. 2,and those of invasive cells to 63 ± 5. 8 and 116. 8 ± 9. 8,respectively(each P< 0. 01). Conclusion KCNMA1 is highly expressed in PC cell lines MiaPaCa-2 and PANC-1,while silencing KCNMA1 gene can effectively inhibit the proliferation,migration and invasion and promote the apoptosis of PC cells.

关 键 词：KCNMA1 胰腺癌 增殖 凋亡 迁移 侵袭 

分 类 号：R735.9[医药卫生—肿瘤]