靶向沉默Zwilch对人皮肤恶性黑素瘤细胞增殖及凋亡的影响  

Effects of targeted silencing Zwilch on proliferation and apoptosis in human cutaneous malignant melanoma cell

作　　者：杨景煜 顾珈榕 郭静[1] 杨瑞[1] 王文成 李云凤 徐平[1] 顾金海 YANG Jingyu;GU Jiarong;GUO Jing;YANG Rui;WANG Wencheng;LI Yunfeng;XU Ping;GU Jinhai(Department of Dermatology,People’s Hospital of Ningxia Hui Autonomous Region,Yinchuan 750002,China;Ningxia Key Laboratory of Cerebrocranial Diseases,Ningxia Medical University,Yinchuan 750004,China;Medical School of Ningbo University,Ningbo 315211,China)

机构地区：[1]宁夏回族自治区人民医院皮肤科,宁夏银川750002 [2]宁夏医科大学颅脑疾病重点实验室,宁夏银川750004 [3]宁波大学医学院,浙江宁波315211

出　　处：《宁夏医学杂志》2021年第5期385-387,共3页Ningxia Medical Journal

基　　金：宁夏自然科学基金项目(2018AAC03268);国家自然科学基金项目(81160312)。

摘　　要：目的探讨靶向沉默Zwilch对人皮肤恶性黑素瘤细胞增殖及凋亡的影响。方法通过感染shRNA慢病毒靶向沉默A375细胞Zwilch的表达及作用,实时荧光定量PCR及Western blotting检测其沉默效果;采用CCK-8和Annexin V/PI染色分别检测靶向沉默Zwilch对A375细胞的增殖及凋亡的影响。结果慢病毒感染72 h后,sh-Zwilch细胞的Zwilch mRNA及蛋白表达水平均显著下降(P<0.05),shRNA慢病毒靶向沉默A375中Zwilch的表达和作用与空白组比较,sh-Zwilch组细胞活性随时间显著下降(P<0.05),凋亡比例明显上升(P<0.05)。结论靶向沉默Zwilch能够有效抑制人皮肤恶性黑素瘤细胞增殖并诱导其凋亡,从而有望成为其基因靶向治疗的一种新靶点和新策略。Objective To investigate the effects of targeted silencing Zwilch on proliferation and apoptosis in human cutaneous malignant melanoma cell.MethodsTargeted silence the expression and effect of Zwilch(sh-Zwilch)in A375 cells was examined by shRNA lentivirus infected.Then the expression levels of Zwilch mRNA and protein in the cell were detected by real-time quantitative PCR and western blotting respectively.The cellular viability was analyzed by CCK-8 assay.The apoptosis was evaluated by Annexin V/PI staining.ResultsCompared with the control cell,A375 cell infected with sh-Zwilch showed significant decrease of expression level of Zwilch mRNA and protein.Subsequently,Zwilch targeted silencing significantly suppressed the cellular viability in a time-dependent manner and increased the apoptosis rate in A375 cell(P<0.05).ConclusionTargeted silence of Zwilch could inhibit the proliferation and promotes the apoptosis of A375 cell,and Zwilch maybe a potential target for human CMM gene therapy.

关 键 词：SHRNA Zwilch 黑素瘤 增殖 凋亡 

分 类 号：R739.5[医药卫生—肿瘤]