基于质谱的DNA加合物组技术在药学领域应用的研究进展  

Research progress in application of mass spectrometry-based DNA adduct technology in field of pharmacy

作　　者：闫明 叶倩 王雪[2] 文海若 YAN Ming;YE Qian;WANG Xue;WEN Hairuo(China Pharmaceutical University,Nanjing 211198,China;National Center for Safety Evaluation of Drugs,National Institutes for Food and Drug Control,Key Laboratory of Beijing for Nonclinical Safety Evaluation Research of Drugs,Beijing 100176,China)

机构地区：[1]中国药科大学,江苏南京211198 [2]中国食品药品检定研究院国家药物安全评价监测中心,药物非临床安全性评价研究北京市重点实验室,北京100176

出　　处：《药物评价研究》2021年第4期876-884,共9页Drug Evaluation Research

基　　金：国家“重大新药创制”科技重大专项(2018ZX09201017-001);中国食品药品检定研究院学科带头人培养基金资助项目(2019X4)。

摘　　要：DNA加合物是评价DNA损伤最直接的分子证据,随之产生一个专门研究DNA修饰识别和DNA加合物定量的新领域——DNA加合物组学。其中质谱(MS)分析法是常用的DNA加合物组学技术,并且随着质谱分析仪的灵敏度和扫描速度的提高,使得利用DNA加合物组学技术来筛查人类基因组中DNA损伤成为可能。其中,基于质谱技术对马兜铃、巴豆醛等致癌物生成的DNA加合物的种类的检测应用十分广泛。主要就基于质谱的加合物组学检测技术及其在致癌物检测中的应用进行概述。DNA adducts are the most direct molecular evidence for evaluating DNA damage,and a new field specializing in DNA modification recognition and DNA adduct quantification—DNA adductomics has emerged.Mass spectrometry(MS)is a commonly used DNA adductomics technology,and with the increase in the sensitivity and scanning speed of mass spectrometry,the use of DNA adductomics technology to screen DNA in the human genome Damage becomes possible.The detection of DNA adducts produced by carcinogens such as aristolochia and crotonaldehyde based on mass spectrometry is very widely used.This article mainly summarizes the adductomics detection technology based on mass spectrometry and its application in the detection of carcinogens.

关 键 词：DNA加合物 加合物组学 质谱 致癌物 

分 类 号：R917[医药卫生—药物分析学]