精氨酸处理对采后冬枣黑斑病的抗病作用  

Effect of Arginine Treatment on Disease Resistance to Alternaria Rot of Postharvest Winter Jujube

作　　者：常璐璐 张乐乐 于有伟 王小佳 张少颖 CHANG Lulu;ZHANG Lele;YU Youwei;WANG Xiaojia;ZHANG Shaoying(College of Food Science,Shanxi Normal University,Linfen 041004,China)

机构地区：[1]山西师范大学食品科学学院,山西临汾041004

出　　处：《食品工业科技》2021年第12期321-326,共6页Science and Technology of Food Industry

基　　金：国家自然科学基金(31671905);山西留学回国人员科技活动择优资助项目(2018)。

摘　　要：为研究精氨酸(Arginine,Arg)处理对采后冬枣果实抗病性的影响,用不同浓度的Arg溶液(0、20、200、1000μmol/L)浸泡枣果实,再对处理后的枣果实进行互隔交链孢(Alternaria alternata)接种,测定贮藏期间病斑直径、抗病相关酶的活性以及黄酮、多酚、木质素的含量。结果表明,Arg处理可以显著减缓冬枣接种A.alternata后病斑直径的扩展,进一步研究表明Arg处理能诱导其苯丙氨酸解氨酶(phenylalamineammonialyase,PAL)、过氧化物酶(peroxidase,POD)、β-1,3-葡聚糖酶(β-1,3-glucanase,GLU)、几丁质酶(chitinase,CHI)活性的上升,其中200μmol/L Arg处理的抗病相关酶活性最高。同时,Arg处理能提高枣果实中黄酮、多酚和木质素的含量,且200μmol/L Arg处理组的黄酮、多酚和木质素含量最高。由此表明,Arg处理通过提高抗病相关蛋白酶(PAL、POD、GLU、CHI)的活性以及黄酮、多酚、木质素的含量来增强采后冬枣果实的抗病能力,有望成为一种新的防腐保鲜剂应用于冬枣果实的防腐保鲜。In order to study the effect of arginine(Arg)treatment on disease resistance of postharvest winter jujube fruit,the fruits were soaked in Arg solutions at different concentrations(0,20,200,1000μmol/L),then Alternariaalternata challenge inoculation was performed on the treated jujube fruits,and later the lesion diameter,the activities of resistance-related enzymes and the contents of flavones,polyphenols and lignin were determined.The results showed that Arg treatment significantly slowed down the expansion of the lesion diameter in the jujube fruitsinoculated with A.alternata.Further studies showed that Arg treatment induced the increase of the activities of phenylalamine ammonialyase(PAL),peroxidase(POD),β-1,3-glucanase(GLU)and chitinase(CHI),among which Arg treatment at 200μmol/L was the most active concentration.At the same time,Arg treatment increased the contents of flavones,polyphenols and lignin in winter jujube,and the contents of flavones,polyphenols and lignin in the fruits treated by 200μmol/L Arg were the highest.Therefore,Arg treatment enhanced the disease resistance of postharvest winter jujube fruits by increasing the activities of diseaseresistant enzymes(PAL,POD,GLU,CHI)and the contents of flavones,polyphenols and lignin,and it can be a promising alternative that will be used in the preservation of postharvest winter jujube.

关 键 词：精氨酸 冬枣 互隔交链孢 抗病性 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]