氨基酸分析仪法快速检测土鳖虫中谷氨酸含量  

Rapid determination of glutamic acid content in Eupolyphaga Steleophaga using an amino acid analyzer

作　　者：张华丽 周洪亮 孙家昌 ZHANG Hua-li;ZHOU Hong-liang;SUN Jia-chang(Collaborative Innovation Center for Birth Defect Research and Transformation of Shandong Province,Jining Medical University, Jining 272067,China;Jining Maternal and Child Health Family Planning Service Center, Jining 272000, China)

机构地区：[1]济宁医学院山东省出生缺陷研究与转化协同创新中心,山东济宁272067 [2]济宁市妇幼保健计划生育服务中心,山东济宁272000

出　　处：《山东科学》2021年第3期26-32,共7页Shandong Science

基　　金：济宁市科技助推新旧动能转换计划(2017SMNS006);山东省中医药科技发展计划(2019-0444)。

摘　　要：采用氨基酸分析仪建立了土鳖虫中谷氨酸含量的检测方法,比较不同浓度的盐酸以及不同料液比、提取时间对土鳖虫中谷氨酸提取效果的影响。结果表明,土鳖虫中谷氨酸的最佳提取工艺为6 mol/L的盐酸消解,料液比1:1(mg/mL),水解时间22 h,建立的谷氨酸分析仪法回收率为99.2%,精密度试验相对标准偏差为0.19%,不同地区土鳖虫中谷氨酸质量分数存在较明显差异,范围为1.068%~1.149%。该方法重现性好、快速高效、操作简便,适用于土鳖虫中氨基酸含量的检测,可为其质量控制及资源深入开发提供技术支持。The effects of various concentrations of hydrochloric acid,material-to-liquid ratios,and extraction times on the extraction of glutamate in Eupolyphaga Steleophaga were compared using an amino acid analyzer to establish a method to measure the glutamate content in Eupolyphaga Steleophaga.The results showed that the best extraction process for glutamic acid in Eupolyphaga Steleophaga was a material-to-liquid ratio of 1:1(mg/mL),6 mol/L hydrochloric acid,and hydrolysis time of 22 h.The recovery rate of the glutamic acid analyzer method was 99.2%,and the relative standard deviation of the precision test was 0.19%.There were significant differences in the glutamate content in Eupolyphaga Steleophaga from different regions,ranging from 1.068%to 1.149%.The method is reproducible,fast,efficient,and easy to perform.It is suitable for the detection of amino acid content in Eupolyphaga Steleophaga and can provide technical support for its quality control and in-depth resource development.

关 键 词：土鳖虫 氨基酸 提取方法 氨基酸分析仪 

分 类 号：R284.2[医药卫生—中药学]