TRAIL与kallistatin联合表达载体的构建及抗肿瘤活性分析  

Construction of TRAIL and Kallistatin Combined Expression Vector and Its Antitumor Activity Analysis

作　　者：王晓[1,2] 黄晓平 刁勇[1,2] WANG Xiao;HUANG Xiaoping;DIAO Yong(School of Medical,Huaqiao University,Quanzhou 362021,China;School of Biomedical Sciences,Huaqiao University,Quanzhou 362021,China)

机构地区：[1]华侨大学医学院,福建泉州362021 [2]华侨大学生物医学学院,福建泉州362021

出　　处：《华侨大学学报（自然科学版）》2021年第3期378-383,共6页Journal of Huaqiao University(Natural Science)

基　　金：国家自然科学基金资助项目(81371669);福建省自然科学基金资助项目(2017J01548);福建省泉州市科技项目(2020C061,2016N070);华侨大学科研基金资助项目(17BS501)。

摘　　要：为研究肿瘤坏死因子相关凋亡诱导配体(TRAIL)与组织激肽释放酶结合蛋白(kallistatin)联合用药的抗肿瘤作用,构建TRAIL与kallistatin双表达的重组质粒pAM-CAG-Kal-IRES-TRAIL,将重组质粒转染A549,LO-2,NCI-H446和Hela细胞,考察其抗肿瘤活性.实验结果表明:构建的双表达载体能同时表达TRAIL与kallistatin,且均能分泌至培养基中;TRAIL与kallistatin联合表达对肿瘤细胞活力的抑制作用明显增强,诱导肿瘤凋亡的作用也明显增强,说明联合表达TRAIL与kallistatin能够增强抗肿瘤活性.In order to study the anti-tumor effect of tumor necrosis factor-related apoptosis-inducing ligand(TRAIL)combined with kallistatin,a dual-expression of TRAIL and kallistatin recombinant plasmid pAM-CAG-Kal-IRES-TRAIL was constructed and then transfected into A549,LO-2,NCI-H446 and Hela cells with liposome to investigate the anti-tumor activity of the dual expression vector.The experimental results show that the constructed dual expression vector can express TRAIL and kallistatin simultaneously,and both of these proteins can be secreted into the culture medium;the combination of TRAIL and kallistatin expression significantly enhances the inhibition of tumor cells viability and induces apoptosis of tumor cells,which indicates that the combined expression of TRAIL and kallistatin can enhance anti-tumor activity.

关 键 词：抗肿瘤 组织激肽释放酶结合蛋白 肿瘤坏死因子相关凋亡诱导配体 细胞凋亡 

分 类 号：R730.54[医药卫生—肿瘤]