表没食子儿茶素没食子酸酯对小鼠耐缺氧及抗疲劳作用的实验研究  

The anti-fatigue and anti-anoxia effects of epigallocatechin-3-gallic acid in mice#

作　　者：宋梦强 陈燕[1] 杨正蓉 李成良 卢镇江 陈熙 肖贤培 郑倩[2] Song Meng-qiang;Chen Yan;Yang Zheng-rong;Li Cheng-liang;Lu Zhen-jiang;Chen Xi;Xiao Xian-pei;Zheng Qian(2017 Clinical Medicine,North Sichuan Medical College,Nanchong 637100,Sichuan,China;Functional Center,School of Basic Medicine,North Sichuan Medical College,Nanchong 637100,Sichuan,China)

机构地区：[1]川北医学院,四川南充637100 [2]川北医学院基础医学院机能中心,四川南充637100

出　　处：《四川生理科学杂志》2021年第4期533-536,共4页Sichuan Journal of Physiological Sciences

基　　金：四川省教育厅重点项目(17ZA0168);南充市科技厅课题(18SXH20224);四川省大学生创新课题(S201910634086)(S201910634086)。

摘　　要：目的:观察表没食子儿茶素没食子酸酯(Epigallocatechin-3-gallate,EGCG)对小鼠的缺氧耐受及抗疲劳作用并初步探讨其机制与抗氧化作用的关系。方法:将小鼠随机分成对照组、低浓度组(EGCG1)和高剂量组(EGCG2),低剂量EGCG每天灌胃10 g·kg-1,高剂量EGCG每天灌胃50 g·kg-1,25d后,采用低张性缺氧、亚硝酸钠中毒性缺氧、急性脑缺血实验,比较各组的小鼠存活时间观察EGCG对缺氧耐受的影响,检测低张性缺氧小鼠肝脏的超氧化歧化酶(Superoxiadeismutase,SOD)活性、丙二醛(Maleicdicdialdehyde,MDA)含量,初步探讨EGCG对小鼠缺氧耐受及抗疲劳的作用及机制。结果:与对照组相比,EGCG1和EGCG2组可以明显延长低张性缺氧、亚硝酸钠中毒性缺氧以及急性缺氧条件下小鼠存活时间,EGCG不同剂量组小鼠负重游泳时间明显延长;且EGCG1与EGCG2提高小鼠肝脏SOD活性,降低肝脏MDA含量(P<0.05)。结论:EGCG具有耐缺氧和抗疲劳作用,其机制可能与增强肝脏SOD的活性,减少MDA含量,从而提高抗氧化活性,减少氧化损伤有关。Objective:To observe the effects of epigallocatechin gallate(EGCG)on hypoxia tolerance and anti-fatigue in mice and to explore the relationship between its mechanism and antioxidation.Methods:Mice were randomly divided into control group,low-concentration group(EGCG1)and high-concentration group(EGCG2).Low-dose EGCG was gavaged at 10g·kg-1 and high-dose EGCG was gavaged at 50g·kg-1 per day for 25 days,and then hypotension hypoxia,sodium nitrite-induced toxic hypoxia and acute cerebral ischemia were used.The survival time of mice in each group was compared to observe the effect of EGCG on hypoxia tolerance,and the activity of superoxide dismutase(SOD)and the content of maleicdicdialdehyde(MDA)in liver of mice with hypotonic hypoxia were detected,and to investigate the effect and mechanism of EGCG on hypoxia tolerance and anti-fatigue in mice.Results:Compared with the control group,EGCG1 and EGCG2 groups could significantlyprolong the survival time of mice under hypotonic hypoxia,sodium nitrite toxic hypoxia and acute hypoxia,and the weight-bearing swimming time of mice in different dose groups of EGCG was significantly prolonged,and EGCG1 and EGCG2 increased the activity of SOD in liver and decreased the content of MDA in liver(P<0.05).Conclusion:EGCG has the effects of anti-hypoxia and anti-fatigue,and its mechanism may be related to increasing the activity of SOD and reducing the content of MDA in liver,so as to increase the activity of antioxidation and reduce oxidative damage.

关 键 词：表没食子儿茶素没食子酸酯 缺氧耐受 存活时间 自由基 

分 类 号：R28[医药卫生—中药学]