Efficacy of Huanglian root decoction(黄连煎剂)on kidney injury in rat’s model of metabolic syndrome  

作　　者：CHEN Xiaoqing ZHANG Yong HUANG Chunlai FU Tingting TAO Qinghua MA Liqiang WANG Liping 

机构地区：[1]Division of Nephrology,the 900th Hospital of Joint Logistic Support Force,PLA,Fuzhou 350025,China

出　　处：《Journal of Traditional Chinese Medicine》2021年第1期117-124,共8页中医杂志（英文版）

基　　金：Supported by the National Natural Science Foundation-funded Project:Coptis Decoction Protects Renal Damage Caused by Metabolic Syndrome(No.81373837);the Natural Science Foundation of Fujian province-funded Project:(the mechanical and clinical approach of the berberine treatment for renal damage caused by metabolic syndrome through regulating IRE1-XBP1 pathway)(No.2018J01184)。

摘　　要：OBJECTIVE:To evaluate the efficacy of Huanglian root decoction(黄连煎剂,HLD)on kidney injury in rat’s model of metabolic syndrome(Met S),and investigate the possible mechanism.METHODS:A fructose-induced Met S rat model and human renal tubular epithelial cell-line model were used to compare the efficacy of HLD with that of berberine and tauroursodeoxycholic acid(TUDCA).Blood pressure,biochemical parameters,histopathological changes and the expression levels of oxidative stress markers were evaluated in the animal model at the end of an 8-week treatment regimen.Oxidative stress markers and molecules of the signal pathway of endoplasmic reticulum(ER)stress were evaluated in the human cell-line model.RESULTS:Levels of fasting insulin,systolic blood pressure and diastolic blood pressure were significantly decreased in rats in the Huanglian group compared to those in the Met S group(P<0.05).Rats treated with HLD and TUDCA exhibited a significant reduction in blood levels of malondialdehyde compared to those in rats in the Met S group(P<0.05).Significant increases in glutathione peroxidase in human tubular epithelial cells was found in the Huanglian group compared to that in the Met S group(14.02 vs 18.31,P<0.05).The m RNA expression of protein kinase RNA-like endoplasmic reticulum kinase and eukaryotic translation initiation factor 2αdecreased significantly in Huanglian groups compared with that in the Met S group.CONCLUSION:HLD has therapeutic efficacy on kidney injury in the Met S rat’s model,and is non-inferior to berberine and TUDCA.

关 键 词：Coptis BERBERINE metabolic syndrome endoplasmic reticulum stress oxidative stress 

分 类 号：R285.5[医药卫生—中药学]