脉冲场凝胶电泳应用于一起肠炎沙门菌引起的食物中毒调查  

Survey on pulsed field gel electrophoresis applied in food poisoning caused by Salmonella enteritidis

作　　者：耿磊 贾肇一[2] 孙汝春 刘晴 王颖童[2] GENG Lei;JIA Zhao-yi;SUN Ru-chun;LIU Qing;WANG Ying-tong(Xinji Center for Disease Control and Prevention,Hebei 052360,China;不详)

机构地区：[1]辛集市疾病预防控制中心,河北052360 [2]河北省疾病预防控制中心,河北省传染病病原鉴定分析与流行病学重点实验室

出　　处：《医学动物防制》2021年第2期149-151,共3页Journal of Medical Pest Control

基　　金：河北省卫生计生委医学科研项目(20170053)。

摘　　要：目的应用脉冲场凝胶电泳(pulsed field gel electrophoresis,PFGE)技术,对一起食物中毒分离的肠炎沙门菌进行同源性分析。方法对患者进行流行病学调查,采集患者和食品样本,依照相关国标和指南进行菌株分离、生化鉴定、血清分型、PFGE检测、图谱聚类分析。结果根据患者流行病学调查结果推测可疑食品为咸鸡蛋。自2例患者粪便和1枚咸鸡蛋中共分离到3株肠炎沙门菌,PFGE图谱条带相似性100%。结论本次疫情是由于食用肠炎沙门菌污染的咸鸡蛋而引起的食物中毒。PFGE技术的应用精准地锁定了传染源头,为流行病学调查提供了有力证据,是对传统方法的有力补充。Objective The homology analysis on Salmonella enteritidis isolated from food poisoning is conducted by use of pulsed field gel electrophoresis(PFGE).Methods Epidemiological survey was carried out,patients and food samples were collected,and the strain isolation,biochemical identification,serotyping,PFGE detection and spectral clustering were implemented according to the relevant national standards and guidelines.Results The suspected food was salted egg based on results of epidemiological survey.Three strains of Salmonella enteritidis were isolated from feces of two patients and one salted egg.The spectral bands of PFGE of such strains were found to be of 100%similarity.Conclusion The food poisoning is caused by Salmonella enteritidis,and the sources of contamination are confirmed to be salted eggs.PFGE technology applied has accurately targeted the source of infection and provided satisfactory evidences for epidemiological survey,being a powerful complement to traditional methods.

关 键 词：肠炎沙门菌 食物中毒 脉冲场凝胶电泳 聚类分析 流行病学 调查 

分 类 号：R378.22[医药卫生—病原生物学] R516.2[医药卫生—基础医学]