小麦黄花叶病抗性鉴定和全基因组关联分析  

Resistance Identification of Wheat Yellow Mosaic Virus and Genome-Wide Association Study

作　　者：郭营[1] 孙俊生 屈春艳 郭宝晋 赵岩[1] 李斯深[1] Guo Ying;Sun Junsheng;Qu Chunyan;Guo Baojin;Zhao Yan;Li Sishen(Shandong Agricultural University/State Key Laboratory of Crop Biology,Taian 271018,China;Zaozhuang College,Zaozhuang 277160,China)

机构地区：[1]山东农业大学/作物生物学国家重点实验室,山东泰安271018 [2]枣庄学院,山东枣庄277160

出　　处：《山东农业科学》2021年第5期51-57,共7页Shandong Agricultural Sciences

基　　金：国家重点研发计划“七大农作物育种”重点专项(2017YFD0100600)。

摘　　要：小麦黄花叶病是我国黄淮麦区一种重要的小麦病害。本研究对134份品种(系)进行小麦黄花叶病抗性鉴定;结合90K SNP芯片基因型分型数据,对小麦黄花叶病病情指数进行全基因组关联分析。结果表明,134份供试材料中,27个品种(系)对小麦黄花叶病表现为高抗,占供试材料的20.15%;21个品种(系)表现中抗,占供试材料的15.67%。通过全基因组关联分析(GWAS),检测到56个SNP标记与小麦黄花叶病病情指数显著关联,分别位于2A、2B、2D、3A、4B、7B染色体上,可解释8.8%~29.6%的表型变异。其中,在2D染色体上检测到在3个环境及其最佳线性无偏预测值(BLUP)中均显著关联的SNP标记有40个,位于76.57~86.28 cM区间。Wheat yellow mosaic caused by wheat yellow mosaic virus(WYMV)is one of the most serious diseases in the Huanghe-Huaihe Wheat Region of China.In the present study,the resistance of 134 varieties(lines)to WYMV was identified and the genome-wide association study(GWAS)was carried out for the disease index(DI)using the 90 K SNP markers.The results showed that 27 varieties were resistant(R),accounting for 20.15%;21 varieties were moderately resistant(MR)to WYMV accounting for 15.67%.A total of 56 SNPs on chromosomes 2 A,2 B,2 D,3 A,4 B and 7 B were detected as associated with WYMV resistance significantly,which could explain 8.8%~29.6%of the phenotypic variations.Among them,40 SNPs were detected on chromosome 2 D under the E1,E2 and E3 environments and the BLUP,and they were located in the region of 76.57~86.28 cM.

关 键 词：小麦黄花叶病 病情指数 SNP 全基因组关联分析 

分 类 号：S512.103.4[农业科学—作物学] Q78[生物学—分子生物学]