HPLC法测定蒙成药三子散中没食子酸的含量  

Determination of gallic acid in Mongolian patent medicine Sanzi powder by HPLC

作　　者：王思文 娜仁图雅 籍学伟 Wang Siwen;NarenTuya;JI Xuewei(Hohhot Institute for Food and Drug Control,Hohhot Inner Mongolia 010000,China;Inner Mongolia Institute For Drug Control,Hohhot Inner Mongolia 010020,China)

机构地区：[1]呼和浩特市食品药品检验所,内蒙古自治区呼和浩特010000 [2]内蒙古自治区药品检验研究院,内蒙古自治区呼和浩特010020

出　　处：《北方药学》2021年第1期21-24,共4页Journal of North Pharmacy

摘　　要：目的:建立蒙成药三子散中没食子酸的含量测定方法。方法:采用HPLC法,采用Phenomenex C18色谱柱(250mm×4.6 mm,5μm),流动相为甲醇-0.1%磷酸水溶液(5∶95),流速1.0 mL(min^(-1)),柱温35℃,进样量为10μL,检测波长为273nm。结果:没食子酸进样量范围在6.357~158.935μg时,进样量与峰面积线性关系良好(r=0.9999);平均回收率为97.70%,RSD为1.30%。结论:该方法简便、灵敏、准确,可用于三子散中没食子酸的测定。Objective:To establish a method for the determination of gallic acid in Mongolian patent medicine Sanzi powder.Methods:The HPLC method was established on a phenomenex C18 column(250 mm×4.6 mm,5μm),the mobile phase was methanol-0.1%phosphoric acid(5:95),the flow rate was 1.0 ml(min^(-1)),the column temperature was 35℃,the injection volume was 10μL,and the detection wavelength was 273nm.Results:The injection volume and peak area of gallic acid had a good linear relationship when the injection volume ranged from 6.357μg to 158.935μg(r=0.999).the average recovery was 97.70%,with RSD was 1.30%.Conclusion:This established mothod is simple,sensitive and accurate,and can be used for the determination of gallic acid in Sanzi powder.

关 键 词：高效液相色谱法 三子散 没食子酸 

分 类 号：R917[医药卫生—药物分析学]