白秃马勃粗脂肪化学成分分析  

Chemical constituents analysis of crude fatty of the puffball Calvatia candida(Rostk.)Hollós

作　　者：李皓 陈浒 朱峰[2,3] 梁滢滢 梁晓棠 陈佳珊 曾玉环 LI Hao;CHEN Xu;ZHU Feng;LIANG Ying-ying;LIANG Xiao-tang;CHEN Jia-shan;ZENG Yu-huan(School of Food Science and Engineering,Foshan University,Foshan 528225,China;Foshan City Engineering&Technology Research Center for Bioactive Natural Products&Functional Chemicals,Foshan University,Foshan 528000,China;School of Environment and Chemical Engineering,Foshan University,Foshan 528000,China)

机构地区：[1]佛山科学技术学院食品科学与工程学院,广东佛山528225 [2]佛山科学技术学院佛山市活性天然产物与功能化学品工程技术研究中心,广东佛山528000 [3]佛山科学技术学院环境与化学工程学院,广东佛山528000

出　　处：《佛山科学技术学院学报（自然科学版）》2021年第3期41-46,共6页Journal of Foshan University(Natural Science Edition)

基　　金：佛山市科技计划项目公共服务能力提升专项(2018AB003721);广东省大学生创新创业训练计划项目(S202011847076)。

摘　　要：采用乙醚连续渗漉提取白秃马勃粗脂肪,利用硅胶柱色谱对白秃马勃粗脂肪化学成分进行分离纯化,通过气相色谱-质谱联用技术(GC-MS)分析菌油脂肪酸成分,运用电喷雾电离质谱(ESI-MS)、一维核磁共振谱(1D NMR)和二维核磁共振谱(2D NMR)解析化合物结构,通过体外实验测试菌油对1,1-二苯基-2-苦基肼基(DPPH)的清除能力。结果表明,白秃马勃菌油主要脂肪酸为亚油酸(44.11%)、油酸(27.49%)和棕榈酸(17.60%)。2个单体化合物被解析为麦角甾-5,8-表二氧-6,22-二烯-3-乙酸酯(MB1)和麦角甾-5,8-表二氧-6,22-二烯-3-醇(MB2)。白秃马勃菌油在试验浓度范围内具有一定的DPPH自由基清除能力,半数清除浓度(IC50)为26.61 mg/mL,并且呈浓度依赖关系。化合物MB1和MB2均为首次从白秃马勃菌分离得到。The crude fat of the puffball Calvatia candida(Rostk.)Hollós was extracted by ether and purified by silica column chromatography.The fatty acid composition of the fungal oil was analyzed by gas chromatography-mass spectrometry(GC-MS),and the compound’s structure was elucidated by electrospray ionization mass spectrometry(ESI-MS),one-dimensional nuclear magnetic resonance spectroscopy(1 D NMR)and two-dimensional nuclear magnetic resonance spectroscopy(2 D NMR),and the scavenging activity of the fungal oil against 1,1-diphenyl-2-bitter hydrazine(DPPH)was tested in vitro.The results show that the main fatty acids in the fungal oil were linoleic acid(44.11%),oleic acid(27.49%)and palmitic acid(17.60%),respectively.The two monomer compounds were elucidated as ergosta-5,8-phenylenedio-6,22-diene-3-yl acetate(MB1)and ergosta-5,8-phenylenedio-6,22-diene-3-ol(MB2),respectively.The fungal oil had DPPH scavenging activity in the range of experimental concentration with the half scavenging concentration(IC50)of 26.61 mg/mL,and showed concentration dependence.Both the compounds MB1 and MB2 were isolated from C.candida for the first time.

关 键 词：白秃马勃 粗脂肪 化学成分 脂肪酸 甾体化合物 1 1-二苯基-2-苦基肼基 

分 类 号：O623.6[理学—有机化学]