A decrease of docosahexaenoic acid in testes of mice fed a high-fat diet is associated with impaired sperm acrosome reaction and fertility  

作　　者：Julio Bunay Luz-Maria Gallardo Jorge Luis Torres-Fuentes M Veronica Aguirre-Arias Renan Orellana Nestor Sepulveda Ricardo D Moreno 

机构地区：[1]Physiology Department,Faculty of Biological Sciences,Pontificia Universidad Cat6lica de Chile(PUC),Santiago 8331150,Chile [2]Department of Chemistry and Biological Sciences,Health Sciences Faculty,Universidad Bernardo 0 Higgins,Santiago 8370854,Chile [3]Center of Excellence in Biotechnology of Reproduction,Universidad de la Frontera,Temuco 4780000,Chile.

出　　处：《Asian Journal of Andrology》2021年第3期306-313,共8页亚洲男性学杂志（英文版）

基　　金：from FONDECYT(1150352);CONICYT(21120505),Chile.

摘　　要：Obesity is a major worldwide health problem that is related to most chronic diseases,including male infertility.Owing to its wide impact on health,mechanisms underlying obesity-related infertility remain unknown.In this study,we report that mice fed a high-fat diet(HFD)for over 2 months showed reduced fertility rates and increased germ cell apoptosis,seminiferous tubule degeneration,and decreased intratesticular estradiol(E2)and E2-to-testosterone ratio.Interestingly,we also detected a decrease in testicular fatty acid levels,behenic acid(C22:0),and docosahexaenoic acid(DHA,22:6n-3),which may be related to the production of dysfunctional spermatozoa.Overall,we did not detect any changes in the frequency of seminiferous tubule stages,sperm count,or rate of in vitro capacitation.However,there was an increase in spontaneous and progesterone-induced acrosomal exocytosis(acrosome reaction)in spermatozoa from HFD-fed mice.These data suggest that a decrease in E2 and fatty acid levels influences spermatogenesis and some steps of acrosome biogenesis that will have consequences for fertilization.Thus,our results add new evidence about the adverse effect of obesity in male reproduction and suggest that the acrosomal reaction can also be affected under this condition.

关 键 词：cholesterol ESTRADIOL fat acid TESTIS TESTOSTERONE 

分 类 号：R285.5[医药卫生—中药学]