NSCLC组织中Ape1/Ref-1及microRNA-21表达与预后关系  

Relationship between the expressions of Ape1/Ref-1 and microRNA-21 in NSCLC tissues and prognosis

作　　者：张海燕[1] 冯平[2] 张洪艳[3] 杨雅婷 路云涛 ZHANG Haiyan;FENG Ping;ZHANG Hongyan;YANG Yating;LU Yuntao(Department of Outpatient,The First Affiliated Hospital of Hebei North University,Zhangjiakou,Hebei,China,075000;Department of Respiratory and Critical Care Medicine,The First Affiliated Hospital of Hebei North University,Zhangjiakou,Hebei,China,075000;Third Department of Neurology,The First Affiliated Hospital of Hebei North University,Zhangjiakou,Hebei,China,075000;Department of Neurosurgery,The First Hospital of Xingtai,Xingtai,Hebei,China,054001)

机构地区：[1]河北北方学院附属第一医院门诊部,河北张家口075000 [2]河北北方学院附属第一医院呼吸与危重症医学科,河北张家口075000 [3]河北北方学院附属第一医院神经内三科,河北张家口075000 [4]邢台市第一医院神经外科,河北邢台054001

出　　处：《分子诊断与治疗杂志》2021年第5期803-806,815,共5页Journal of Molecular Diagnostics and Therapy

基　　金：河北省医学科学研究课题计划资助项目(20150857)。

摘　　要：目的探讨非小细胞肺癌(NSCLC)组织中脱嘌呤脱嘧啶核酸内切酶/氧化还原因子-1(Ape1/Ref-1)及微小RNA-21(microRNA-21)表达与化疗疗效及预后的关系。方法选取2015年10月至2017年10月期间本院78例晚期NSCLC化疗患者组织及癌旁组织,均接受NSCLC标准一线化疗方案。比较Ape1/Ref-1、microRNA-21的表达水平,根据化疗疗效分为有效组、无效组。比较癌灶组织与癌旁组织、有效组与无效组Ape1/Ref-1、microRNA-21表达情况,采用Spearman相关性分析化疗疗效与癌灶组织Ape1/Ref-1、microRNA-21表达水平的相关性。Kaplan-Meier法分析患者总生存期(OS)与癌灶组织Ape1/Ref-1、microRNA-21表达的相关性,COX回归模型分析影响生存率的独立相关因素。结果癌灶组织Ape1/Ref-1高表达率及microRNA-21相对表达量均高于癌旁组织,有效组癌灶组织Ape1/Ref-1、microRNA-21高表达率均低于无效组,差异均有统计学意义(P<0.05)。化疗疗效与癌灶组织Ape1/Ref-1、microRNA-21表达均呈负相关(P<0.05)。Ape1/Ref-1、microRNA-21低表达患者3年生存率均高于高表达患者,差异均有统计学意义(P<0.05)。癌灶组织Ape1/Ref-1、microRNA-21表达是NSCLC患者长期生存的独立相关因素(P<0.05)。结论NSCLC癌灶组织中Ape1/Ref-1、microRNA-21的表达与化疗疗效及预后密切相关。Objective To investigate the relationship between the expression of apurinic/aprimidinic endonuclease/redox factor-1(Ape1/Ref-1)and microRNA-21 in non-small cell lung cancer(NSCLC)tissues and chemotherapy efficacy and prognosis.Methods A total of 78 patients with advanced NSCLC undergoing chemotherapy were included and received NSCLC standard first-line chemotherapy.Immunohistochemistry was used to detect the expression levels of Ape1/Ref-1 and microRNA-21 in cancer tissues and adjacent tissues in pathological samples obtained before chemotherapy.According to the chemotherapy efficacy,the patients were divided into the effective group and the ineffective group.The expressions of Ape1/Ref-1 and microRNA-21 were compared between cancer tissues and adjacent tissues and between the effective group and the ineffective group.Spearman correlation analysis was used to analyze the correlation between chemotherapy efficacy and the expression levels of Ape1/Ref-1 and microRNA-21 in cancer tissues.Kaplan-Meier method was used to analyze the correlation between overall survival(OS)and expressions of Ape1/Ref-1 and microRNA-21 in cancer tissues.COX regression model analysis was used to analyze the independent related factors affecting the survival rate.Results The high expression rate of Ape1/Ref-1 and the relative expression of microRNA-21 in cancer tissues were higher than those in adjacent tissues,and the high expression rates of Ape1/Ref-1 and microRNA-21 in cancer tissues in the effective group were lower than those in the ineffective group(P<0.05).Chemotherapy efficacy was negatively correlated with the expressions of Ape1/Ref-1 and microRNA-21 in cancer tissues(P<0.05).The 3-year survival rate of patients with low expression of Ape1/Ref-1 was higher than that of patients with high expression of Ape1/Ref-1,and the 3-year survival rate of patients with low expression of microRNA-21 was higher than that of patients with high expression of microRNA-21(P<0.05).Expressions of Ape1/Ref-1 and microRNA-21 in cancer tissues were

关 键 词：脱嘌呤脱嘧啶核酸内切酶/氧化还原因子-1 微小RNA-21 非小细胞肺癌 

分 类 号：R734.2[医药卫生—肿瘤]