CD90过表达影响胃癌细胞AGS的干性特征  

CD90 Overexpression Affects The Stem Characteristics of Gastric Cancer Cell Line AGS

作　　者：周自华[1] 岳春雪 梁琳 高璐 贺正希 贺军宇 金曦 高梦祥 李欣[3] 周艳宏 ZHOU Zi-Hua;YUE Chun-Xue;LIANG Lin;GAO Lu;HE Zheng-Xi;HE Jun-Yu;JIN Xi;GAO Meng-Xiang;LI Xin;ZHOU Yan-Hong(Department of Oncology,Loudi Central Hospital,Loudi 417000,China;Cancer Research Institute,Basic School of Medicine,Central South University,Changsha 410011,China;Breast Cancer Center,Department of General Surgery,Xiangya Hospital,Central South University,Changsha 410008,China)

机构地区：[1]湖南省娄底市中心医院肿瘤科,娄底417000 [2]中南大学基础医学院,肿瘤研究所,长沙410011 [3]中南大学湘雅医院普外科乳腺肿瘤中心,长沙4100078

出　　处：《生物化学与生物物理进展》2021年第5期580-589,共10页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金(81672685)资助项目。

摘　　要：2019年中国癌症报告显示,胃癌发病率仅次于肺癌,位列第二,其死亡率排在所有肿瘤的第三位,严重危害人们健康.筛查和鉴定胃癌的早期检测标志物、寻找胃癌治疗的分子靶点,对于降低胃癌致死率至关重要.CD90(THY1)是一种细胞表面糖蛋白,在肿瘤细胞增殖、转移以及血管生成中发挥重要作用.CD90异常表达与干细胞特性有关,促进肿瘤的起始与转移.但CD90与胃癌细胞干性特征之间的关系尚未见文献报道.本研究发现,CD90过表达增加胃癌细胞AGS的侧群细胞(SP细胞)比例,影响其膜表面ABCG2、CD105等干性标记物的蛋白质表达水平,同时也影响胃癌细胞AGS胞内NANOG、SOX2等干性标记物的m RNA表达水平,mRNA芯片和Western-blot技术揭示CD90过表达可能通过调控PI3K/Akt和JNK/ERK1/2信号通路活性,影响胃癌细胞AGS的干性特征.本实验结果为胃癌潜在分子靶点的鉴定提供了新的思路.In 2019,the national cancer report showed that the incidence rate of gastric cancer(GC)ranked second,only after lung cancer.Its mortality rate ranked third in all tumors.It seriously endangers people’s health.It is very important for reducing the mortality rate of GC to screen and identify early detection markers and search for molecular targets for its treatment.CD90(THY1)is a cell surface glycoprotein,which plays an important role in tumor cell proliferation,metastasis and angiogenesis.The abnormal expression of CD90 is related to the stem characteristics of cells and promotes the initiation and metastasis of tumor.However,the relationship between CD90 and the stem characteristics of GC cells has not been reported.In this study,we found that CD90 overexpression increased the proportion of side population cells(SP cells)of GC cells AGS.It affected the protein expression levels of stem markers such as ABCG2 and CD105 on the membrane surface,and also affected the m RNA expression levels of stem markers such as NANOG,and SOX2 in GC cells AGS.m RNA microarray and Western blot revealed that CD90 overexpression affected the stem characteristics of GC cells through activating the signal pathway of PI3 K/Akt and JNK/ERK1.Our results provide a new idea for the identification of potential molecular targets of gastric cancer.

关 键 词：CD90基因 胃癌 侧群细胞 干性标志物 分子机制 

分 类 号：R735.2[医药卫生—肿瘤]