蓝萼甲素对牙龈卟啉单胞菌体外抑菌活性研究  

A study on the antibacterial activity of glaucocalyxin A against Porphyromonas gingivalis in vitro

作　　者：杨钰婷 宋忠臣[2] 张旭[3] 周薇[3] 周鲁先 宋莉[1] YANG Yuting;SONG Zhongchen;ZHANG Xu;ZHOU Wei;ZHOU Luxian;SONG Li(Department of Stomatology,The Second Affiliated Hospital of Nanchang University,Institute of Periodontal Disease,Nanchang University,330000,China;Department of Periodontology,Shanghai Ninth People's Hospital,College of Stomatology,Shanghai Jiaotong University School of Medicin,National Clinical Research Center for Oral Disease,Shanghai Key Laboratory of Stomatology;Shanghai Ninth People's Hospital,College of Stomatology,Shanghai Jiao Tong University School of Medicin,Laboratory of Oral Microbiota and Systemic Diseases,National Clinical Research Center for Oral Disease,Shanghai Key Laboratory of Stomatology&Shanghai Research Institute of Stomatology;Shanghai Archgene Biotechnology Co.,Ltd)

机构地区：[1]南昌大学第二附属医院口腔科,南昌大学牙周病研究所,330000 [2]上海交通大学医学院附属第九人民医院·口腔医学院牙周病科,国家口腔疾病临床研究中心,上海市口腔医学重点实验室 [3]上海交通大学医学院附属第九人民医院·口腔医学院,口腔微生态与系统性疾病实验室,国家口腔疾病临床研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所 [4]上海艾济生物科技有限公司

出　　处：《实用口腔医学杂志》2021年第3期317-322,共6页Journal of Practical Stomatology

基　　金：2021年度国家地区基金(编号:82060203);2019年度江西省教育厅重点项目(编号:GJJ180010);2020年度江西省星火计划(编号:20208032)。

摘　　要：目的:探讨蓝萼甲素(GLA)对牙龈卟啉单胞菌(P.gingivalis)的体外抑菌活性。方法:采用肉汤微量稀释法检测GLA对P.gingivalis的最小抑菌浓度(MIC)及最小杀菌浓度(MBC);采用结晶紫染色法及MTT法检测GLA对P.gingivalis生物膜的形成及其代谢活性的影响。结果:GLA对P.gingivalis的MIC值为5μmol/L,MBC值为20μmol/L,1/2 MIC时GLA对P.gingivalis浮游菌的生长即有显著的抑制作用。从1.25μmol/Lol/L开始GLA能够同时抑制P.gingivalis生物膜的形成和代谢活性,当GLA浓度达到5μmol/L时能抑制76%的P.gingivalis生物膜的形成和74%的生物膜代谢活性。结论:GLA在体外对P.gingivalis浮游菌及生物膜的生长均表现出较好的抑制作用,有望成为一种安全有效的牙周辅助治疗药物。Objective:To investigate the antibacterial activity of glaucocalyxin A(GLA)against Porphyromonas gingivalis(P.gingivalis)in vitro.Methods:The minimum inhibitory concentration(MIC)and minimal bactericidal concentration(MBC)of GLA to P.gingivalis were determined by broth microdilution assay.The effects of GLA on the formation and viability of P.gingivalis biofilm were detected by MTT assay and crystal violet staining.Results:The MIC of GLA to P.gingivalis was 5μmol/L,and the MBC was 20μmol/L.GLA at the concentration of 1/2 MIC could inhibit the growth of P.gingivalis obviously.The formation and viability of P.gingivalis biofilm were inhibited by GLA of 1.25μmol/L.GLA of 5μmol/L inhibited P.gingivalis biofilm formation by 76%and biofilm viabilityby 74%.Conclusion:GLA can inhibit the growth and viability of P.gingivalis both in bacterial planktonic cultures and biofilm formation in vitro.It may be a safe and effective drug to periodontitis.

关 键 词：蓝萼甲素 牙龈卟啉单胞菌 微生物敏感性实验 生物膜 

分 类 号：R781.4[医药卫生—口腔医学]