口腔白斑病癌变的血管生成基因芯片检测及表达验证  

Detection of angiogenic genes of oral leukoplakia cancerization by transcriptome array and verification

作　　者：吴苏宁 杨溪[2] 刘伟[2] WU Suning;YANG Xi;LIU Wei(Department of Stomatology,Jiangnan University Affiliated the Third People's Hospital,Jiangsu Province,Wuxi 214000,China;Department of Oral and Maxillofacial-Head and Neck Oncology,Shanghai Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,National Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stomatology&Shanghai Research Institute of Stomatology)

机构地区：[1]江南大学附属医院(第三人民医院)口腔科,无锡214000 [2]上海交通大学医学院附属第九人民医院·口腔医学院口腔颌面头颈肿瘤科,国家口腔疾病临床研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所

出　　处：《实用口腔医学杂志》2021年第3期337-341,共5页Journal of Practical Stomatology

基　　金：国家自然科学基金(编号:82074502)。

摘　　要：目的:研究口腔白斑发生发展中的血管生成基因表达谱,并验证关键基因表达。方法:对正常黏膜(n=3),低危白斑(n=4),高危白斑(n=4),早期鳞癌(n=6)进行转录组学芯片检测生物学作用为血管生成的相关基因,对4个关键基因表达进行了验证。结果:从高危白斑发展到鳞癌有151个基因,为全部异常表达基因占比18.9%。RT-qPCR验证结果显示VEGFA、PDGFRB、MMP1和MMP3在正常黏膜、口腔白斑和鳞癌中的表达量均呈阶梯式升高,与芯片结果基本一致。结论:血管生成基因的异常表达与白斑癌变关系密切。Objective:To study the expression profile of angiogenic genes in the development of oral leucoplakia(OLK)and to verify the expression of key genes.Methods:The angiogenic genes of OLK in normal mucosa(n=3),low risk OLK(n=4),high risk OLK(n=4)and early OSCC(n=6)samples were detected by transeriprome microarray and the expression of 4 key genes were verified by RT-qPCR.Results:There were 151 differentially expressed genes from high risk OLK to OSCC,accounted for 18.9%of all abnormal genes.qRT-PCR results showed that the expression levels of VEGFA,PDGFRB,MMP1 and MMP3 in normal mucosa,OLK and OSCC increased in turn and inkeep with the results of microarray.Conclusion:Abnormal expression of angiogenic genes is closely related to oral carcinogenesis.

关 键 词：口腔白斑 鳞癌 血管生成 VEGFA 异常增生 

分 类 号：R781.5[医药卫生—口腔医学]