卵巢癌患者T细胞、微血管密度与癌细胞转移预后的关系  

Relationship between T cell, microvessel density and metastasis prognosis in patients with ovarian cancer

作　　者：刘小丽[1] 杨志明[2] 王黎黎[1] 马华[1] 王静[3] LIU Xiaoli;YANG Zhiming;WANG Lili;MA Hua;WANG Jing(Department of Obstetrics and Gynecology,North Hospital,General Hospital of Jizhong Energy Fengfeng Group,Handan Hebei 056201;Department of Laboratory Medicine,Handan Central Hospital,Handan Hebei 056200;Department of Obstetrics and Gynecology,First Affiliated Hospital of Hebei North University,Zhangjiakou Hebei 057000,P.R.China)

机构地区：[1]冀中能源峰峰集团总医院北院区妇产科,河北邯郸056201 [2]邯郸市中心医院检验科,河北邯郸056200 [3]河北北方学院附属第一医院妇产科,河北张家口057000

出　　处：《中国计划生育和妇产科》2021年第5期85-88,96,共5页Chinese Journal of Family Planning & Gynecotokology

基　　金：河北省重点科技研究计划项目(项目编号:20181734)。

摘　　要：目的研究卵巢癌患者T细胞、微血管密度(microvessel density, MVD)与癌细胞转移预后的关系。方法选择2017年1~12月河北省冀中能源峰峰集团总医院65例卵巢癌患者为卵巢癌组,30例良性卵巢瘤患者为良性卵巢瘤组,卵巢正常者35例为对照组,流式细胞术检测外周血中CD3^(+)、CD4^(+)、CD8^(+)T淋巴细胞的占比以及计算CD4^(+)/CD8^(+)的比值;以CD34抗原标记卵巢癌组织中的MVD;对卵巢癌患者术后进行回访,并根据结果绘制Kaplan-Meier患者预后生存曲线;Cox单因素和多因素分析影响卵巢癌患者预后的影响因素。结果卵巢癌组患者的血清中T淋巴细胞CD3^(+)、CD4^(+)的占比以及CD4^(+)/CD8^(+)的比值较对照组显著降低,CD8^(+)显著升高,差异有统计学意义(P<0.05);卵巢癌组织和良性卵巢瘤组织中MVD较正常卵巢组织中显著上升(P<0.05);癌细胞远处转移的卵巢癌患者较未转移患者的CD3^(+)、CD4^(+)和CD4^(+)/CD8^(+)显著降低,CD8^(+)和MVD显著上升(P<0.05);T细胞高水平较低水平患者总生存期显著上升(P<0.05);MVD低表达较高表达患者总生存期显著升高(P<0.05);临床分期Ⅲ~Ⅳ期、T细胞低水平以及MVD高水平是影响卵巢癌患者预后的独立危险因素(P<0.05)。结论卵巢癌患者T细胞、MVD与癌细胞转移及预后关系密切,有望用于卵巢癌临床治疗方案选择及预后评估。Objective To study the relationship between T cell, microvessel density(MVD) and metastasis prognosis in patients with ovarian cancer.Methods From January to December 2017,65 patients with ovarian cancer in the Fengfeng Group General Hospital of Hebei Province Jizhong Energy Group were selected as the ovarian cancer group, 30 patients with benign ovarian tumors were selected as the benign ovarian tumor group, and 35 patients with normal ovaries were selected as the control group, the proportion of CD3^(+),CD4^(+) and CD8^(+)T lymphocytes in peripheral blood was detected by flow cytometry and ratio of CD4^(+)/CD8^(+) was calculated.CD34 antigen was used to label MVD in ovarian cancer tissues;follow-up visits were made to ovarian cancer patients after surgery, and Kaplan-Meier patient survival curves were drawn based on the results;Cox univariate and multivariate analysis was used to analyze influencing factors on the prognosis of ovarian cancer patients.Results The percentage of CD3^(+) and CD4^(+) of T lymphocytes and the ratio of CD4^(+)/CD8^(+) in serum of patients with ovarian cancer were significantly lower than control group, and CD8^(+) were higher than normal ovarian tissues, MVD in ovarian cancer and benign ovarian tumor tissues was higher than normal ovarian tissues(P<0.05).CD3^(+),CD4^(+) and CD4^(+)/CD8^(+) of ovarian cancer patients with distant metastasis were lower than non-metastatic ovarian cancer patients.CD8^(+) and MVD were increased in ovarian cancer patients with distant metastasis compared with non-metastatic.Overall survival(OS) was significantly increased in patients with high levels of T cell and low MVD expression.Clinical stage Ⅲ~Ⅳ,low level of T cells and high level of MVD were independent risk factors for prognosis of patients with ovarian cancer(P<0.05).Conclusion T cell and MVD are closely related to cancer cell metastasis and prognosis in patients with ovarian cancer, which is expected to be used to determine the clinical treatment and evaluate the prognosis of ovarian cancer.

关 键 词：卵巢癌 T细胞 微血管密度 转移 预后 

分 类 号：R737.31[医药卫生—肿瘤]