Lycium barbarum extract promotes M2 polarization and reduces oligomeric amyloid-β-induced inflammatory reactions in microglial cells  

作　　者：Zhong-Qing Sun Jin-Feng Liu Wei Luo Ching-Hin Wong Kwok-Fai So Yong Hu Kin Chiu 

机构地区：[1]Department of Ophthalmology,Li Ka Shing Faculty of Medicine,The University of Hong Kong,Hong Kong Special Administrative Region,China [2]State Key Laboratory of Brain and Cognitive Sciences,The University of Hong Kong,Hong Kong Special Administrative Region,China [3]Guangdong-Hong Kong-Macao Institute of CNS Regeneration,Jinan University,Guangzhou,Guangdong Province,China [4]Department of Orthopaedics&Traumatology,The University of Hong Kong,Hong Kong Special Administrative Region,China

出　　处：《Neural Regeneration Research》2022年第1期203-209,共7页中国神经再生研究（英文版）

基　　金：supported by Midstream Research Program for Universities;Hong Kong Special Administrative Region,China,No.MRP-092-17X。

摘　　要：Lycium barbarum(LB)is a traditional Chinese medicine that has been demonstrated to exhibit a wide variety of biological functions,such as antioxidation,neuroprotection,and immune modulation.One of the main mechanisms of Alzheimer’s disease is that microglia activated by amyloid beta(Aβ)transform from the resting state to an M1 state and release pro-inflammatory cytokines to the surrounding environment.In the present study,immortalized microglial cells were pretreated with L.barbarum extract for 1 hour and then treated with oligomeric Aβfor 23 hours.The results showed that LB extract significantly increased the survival of oligomeric Aβ-induced microglial cells,downregulated the expression of M1 pro-inflammatory markers(inducible nitric oxide synthase,tumor necrosis factorα,interleukin-6,and interleukin-1β),and upregulated the expression of M2 anti-inflammatory markers(arginase-1,chitinase-like protein 3,and interleukin-4).LB extract also inhibited the oligomeric Aβ-induced secretion of tumor necrosis factorα,interleukin-6,and interleukin-1βin microglial cells.The results of in vitro cytological experiments suggest that,in microglial cells,LB extract can inhibit oligomeric Aβ-induced M1 polarization and concomitant inflammatory reactions,and promote M2 polarization.

关 键 词：Alzheimer’s disease amyloid-β anti-inflammatory factors Lycium barbarum extract M1 microglia M2 microglia NEUROINFLAMMATION proinflammatory factors 

分 类 号：R453[医药卫生—治疗学] R741.05[医药卫生—临床医学]