槟榔多酚提取物对α-葡萄糖苷酶活性的抑制动力学研究  

Inhibition Kinetics of Polyphenol Extracts from Areca Nut onα-Glucosidase Activity

作　　者：宋菲[1,2] 陈开健 唐敏敏 陈华[1,2] 李永东 SONG Fei;CHEN Kaijian;TANG Minmin;CHEN Hua;LI Yongdong(Coconut Research Institute,Chinese Academy of Tropical Agricultural Sciences,Wenchang,Hainan 571339,China;Hainan Engineering Research Center of Arecanut Industry,Wenchang,Hainan 571339,China;College of Food Science and Engineering,Hainan Tropical Ocean University,Sanya,Hainan 572022,China;Hainan Yulin Coconut Technology Development Co.,Ltd.,Wenchang,Hainan 571339,China)

机构地区：[1]中国热带农业科学院椰子研究所,海南文昌571339 [2]海南省槟榔产业工程研究中心,海南文昌571339 [3]海南热带海洋学院食品科学与工程学院,海南三亚572022 [4]海南雨林椰创科技开发有限公司,海南文昌571339

出　　处：《热带作物学报》2021年第5期1455-1461,共7页Chinese Journal of Tropical Crops

基　　金：海南省自然科学基金面上项目(No.219MS080);中国热带农业科学院基本科研业务费专项(No.1630152017017,No.1630152020007);农业农村部农业科技创新与推广项目“槟榔食用安全性研究及健康新产品开发与示范”。

摘　　要：为了研究槟榔多酚提取物对α-葡萄糖苷酶活性的影响,以75%乙醇为提取溶剂,利用超声波辅助法提取槟榔多酚(槟榔籽和槟榔壳多酚),采用S-8大孔树脂对粗提物进行纯化,探讨纯化前后的槟榔多酚提取物对α-葡萄糖苷酶活性的抑制作用,通过动力学实验判断对酶活性的抑制类型,同时对添加不同浓度槟榔多酚提取物的α-葡萄糖苷酶的荧光光谱和同步荧光光谱进行分析。结果表明:槟榔籽多酚提取物纯化前后的IC50分别为(0.34±0.12)、(0.33±0.10)μg/mL,槟榔壳多酚提取物纯化前后的IC50分别为(1.73±0.31)、(0.14±0.09)mg/mL,纯化后的槟榔壳多酚提取物对α-葡萄糖苷酶活性的抑制作用显著增强。动力学实验表明,槟榔多酚提取物对α-葡萄糖苷酶活性的抑制作用类型为竞争性与非竞争性的混合型抑制。荧光光谱和同步荧光光谱分析结果显示,槟榔多酚提取物对α-葡萄糖苷酶内源性荧光具有淬灭作用,但对酶的构象没有影响。槟榔籽和槟榔壳多酚提取物对α-葡萄糖苷酶活性的抑制作用均较强,可为天然α-葡萄糖苷酶抑制剂的开发提供参考。Areca seed and areca shell polyphenols were extracted using the ultrasonic assisted method with 75%ethanol as extraction solvent and purified by S-8 macropore resin.The inhibitory effect of polyphenol extracts from areca nut onα-glucosidase activity before and after purification was studied.The inhibitory type onα-glucosidase activity was determined by kinetic experiments,and the fluorescence spectrum and synchronous fluorescence spectrum ofα-glucosidase with different concentrations of areca nut polyphenol extracts were analyzed.The results showed that the IC50 of areca seed polyphenol extracts before and after purification was(0.34±0.12)and(0.33±0.10)μg/mL,respectively,and the IC50 of areca shell polyphenol extracts before and after purification was(1.73±0.31)and(0.14±0.09)mg/mL,respectively,indicating that the inhibition onα-glucosidase activity was enhanced after purification of areca shell polyphenol extracts.Kinetic experiments showed that the inhibition of areca nut polyphenol extracts onα-glucosidase activity belonged to the mixed type of competition and non-competition.The fluorescence spectrum and synchronous fluorescence spectrum analysis showed that areca nut polyphenol extracts had quenching effect on the endogenous fluorescence ofα-glucosidase,but had no effect on the conformation ofα-glucosidase.In conclusion,areca seed and areca shell polyphenol extracts have good inhibitory effect onα-glucosidase activity,which could provide reference for the development of natural inhibitors ofα-glucosidase.

关 键 词：槟榔多酚 纯化 Α-葡萄糖苷酶 活性抑制 动力学 荧光淬灭 

分 类 号：S792.91[农业科学—林木遗传育种]